Price D R G, Bell H A, Hinchliffe G, Fitches E, Weaver R, Gatehouse J A
University of Durham, UK.
Insect Mol Biol. 2009 Apr;18(2):195-202. doi: 10.1111/j.1365-2583.2009.00864.x.
Three genes encoding clan MB metalloproteinases (EpMP1-3) were identified from venom glands of the ectoparasitic wasp Eulophus pennicornis. The derived amino acid sequences predict mature proteins of approximately 46 kDa, with a novel two-domain structure comprising a C-terminal reprolysin domain, and an N-terminal domain of unknown function. EpMP3 expressed as a recombinant protein in Pichia pastoris had gelatinase activity, which was inhibited by EDTA. Injection of recombinant EpMP3 into fifth instar Lacanobia oleracea (host) larvae resulted in partial insect mortality associated with the moult to sixth instar, with surviving insects showing retarded development and growth. EpMP3 is expressed specifically in venom glands. These results suggest that EpMP3 is a functional component of Eulophus venom, which is able to manipulate host development.
从外寄生黄蜂细角尤洛飞虱的毒腺中鉴定出三个编码MB家族金属蛋白酶的基因（EpMP1 - 3）。推导的氨基酸序列预测成熟蛋白约为46 kDa，具有一种新颖的双结构域结构，包括一个C端类解聚素结构域和一个功能未知的N端结构域。在毕赤酵母中作为重组蛋白表达的EpMP3具有明胶酶活性，该活性被EDTA抑制。将重组EpMP3注射到五龄甘蓝夜蛾（宿主）幼虫中，导致部分昆虫死亡，这与蜕皮至六龄有关，存活的昆虫表现出发育和生长迟缓。EpMP3在毒腺中特异性表达。这些结果表明，EpMP3是细角尤洛飞虱毒液的一种功能成分，能够操纵宿主的发育。
