Laboratory of Environmental Pollution Analysis, Faculty of Biology, University of Warsaw, Warsaw, Poland.
J Appl Microbiol. 2009 Sep;107(3):858-66. doi: 10.1111/j.1365-2672.2009.04261.x. Epub 2009 Mar 23.
The aim of this study was the isolation and characterization of micro-organisms from Lubin copper mine potentially useful in biotechnology of metal recovery from copper bearing black shale.
Eight bacterial strains were isolated from black shale ore. Phylogenetic analysis based on 16S rRNA gene homology showed that five strains belonged to the gamma-Proteobacteria, one to the Firmicutes and two to the Actinobacteria. The ability of the isolates to transform bituminous shale and use them as carbon and energy sources, as well as high resistance to metals and metalloids, esterase and lipase activities, assimilation of organic acids, degradation of phenanthrene and siderophores production were shown.
The indigenous bacteria exhibited a broad range of physiological properties related to geochemical parameters of the examined environment and potentially useful in biometallurgical procedures.
The results have yielded new insights into the microbiology of black shale. It can be suggested that isolated micro-organisms might play a role in the geochemical cycle of carbon and metals occurring in the organic fraction of black shale ore and might be of potential use in biotechnological procedures for the copper recovery and other valuable metals from tailings containing black shale as well as organic rich ore.
本研究的目的是从卢宾铜矿中分离和鉴定具有从含铜黑色页岩中回收金属的生物技术潜力的微生物。
从黑色页岩矿石中分离出 8 株细菌。基于 16S rRNA 基因同源性的系统发育分析表明，其中 5 株属于γ变形菌，1 株属于厚壁菌门，2 株属于放线菌。这些分离物具有转化沥青页岩并将其用作碳和能源来源的能力，对金属和类金属具有高抗性，酯酶和脂肪酶活性，有机酸同化，菲降解和铁载体生产。
本土细菌表现出与所研究环境的地球化学参数相关的广泛生理特性，在生物冶金过程中具有潜在用途。
该研究结果深入了解了黑色页岩的微生物学。可以认为，分离出的微生物可能在黑色页岩矿石有机质中碳和金属的地球化学循环中发挥作用，并可能在从含有黑色页岩的尾矿以及富含有机物的矿石中回收铜和其他有价值金属的生物技术过程中具有潜在用途。
