Wagner D S, Salari A, Gage D A, Leykam J, Fetter J, Hollingsworth R, Watson J T
Department of Chemistry, Michigan State University, East Lansing 48824.
Biol Mass Spectrom. 1991 Jul;20(7):419-25. doi: 10.1002/bms.1200200705.
Novel and simple procedures for preparing ethyl-triphenylphosphonium derivatives of peptides are described. These procedures allow an ethyl-triphenylphosphonium moiety to be selectively attached to either the N- or C-terminus. The resulting derivatives contain a positive charge at a fixed position and have significant hydrophobic character. Modification of peptides by these chemical methods significantly enhances the efficiency of fast atom bombardment ionization, especially of hydrophilic peptides. Moreover, upon collisionally activated dissociation, the derivatized peptides generate a predictable series of sequence ions from either the C-terminus or the N-terminus, depending on the location of the ethyl-triphenylphosphonium moiety.
描述了制备肽的乙基三苯基鏻衍生物的新颖且简单的方法。这些方法可使乙基三苯基鏻部分选择性地连接到N端或C端。所得衍生物在固定位置带有正电荷，并具有显著的疏水特性。通过这些化学方法对肽进行修饰可显著提高快速原子轰击电离的效率，尤其是对于亲水性肽。此外，在碰撞激活解离时，根据乙基三苯基鏻部分的位置，衍生化的肽会从C端或N端产生一系列可预测的序列离子。
