[人氟斑牙体外再矿化的研究]

[Study on remineralization of human fluorosed teeth in vitro].

作者信息

Luo Li-Ya, Wang Yong, Li Hong, Zheng Hui, Gao Si-Ji

机构信息

State Key Laboratory of Oral Diseases, Sichuan University, Chengdu 610041, China.

出版信息

Hua Xi Kou Qiang Yi Xue Za Zhi. 2009 Feb;27(1):96-9.

PMID:19323407

Abstract

OBJECTIVE

To investigate the transformation of microhardness and microstructures of human dental fluorosis after demineralization and remineralization in vitro.

METHODS

Forty human dental fluorosis enamel blocks were demineralized by acid-etching gel, then subjected to 1.0% casein phosphopeptides stabilized calcium phosphate (CPP-CP) for remineralization. Surface enamel microhardness was measured on the enamel blocks before and after demineralization and after remineralization. The enamel specimens was observed by stereomicroscope and scanning electron microscope, then sectioned (100 microm) and examined by transmission light microscope and polarized light microscope.

RESULTS

Before demineralization, the average knoop hardness value of the fluorosed teeth was (241.53 +/- 21.31) kHV. After demineralization, obvious decrease of the surface hardness of the enamel was observed, the mean value was (175.76 +/- 24.99) kHV (P < 0.05), the percent of the surface microhardness demineralization (% SMHD) was (27.23 +/- 1 4.79)%, and major demineralization was under the surface of the enamel, which was similar to the early natural enamel caries. After remineralization, obvious increase of the surface hardness of the enamel was observed, the mean value was (210.17 +/- 21.48) kHV (P < 0.05), the percent of the surface microhardness remineralization (% SMHR) was (52.32 +/- 4.23)%, major remineralization was under the surface of the enamel.

CONCLUSION

Remineralization could be used to prevent and cure the early natural enamel caries of fluorosed dental teeth.

摘要

目的

研究体外脱矿再矿化后人类氟斑牙的显微硬度和微观结构变化。

方法

40个人类氟斑牙釉质块用酸蚀凝胶脱矿，然后用1.0%酪蛋白磷酸肽稳定磷酸钙（CPP-CP）进行再矿化。在脱矿前、脱矿后及再矿化后测量釉质块表面釉质显微硬度。用体视显微镜和扫描电子显微镜观察釉质标本，然后切片（100微米），并用透射光显微镜和偏光显微镜检查。

结果

脱矿前，氟斑牙的平均努氏硬度值为（241.53±21.31）kHV。脱矿后，观察到釉质表面硬度明显降低，平均值为（175.76±24.99）kHV（P<0.05），表面显微硬度脱矿百分比（%SMHD）为（27.23±14.79）%，主要脱矿发生在釉质表面下，与早期自然釉质龋相似。再矿化后，观察到釉质表面硬度明显增加，平均值为（210.17±21.48）kHV（P<0.05），表面显微硬度再矿化百分比（%SMHR）为（52.32±4.23）%，主要再矿化发生在釉质表面下。