Department of Preventive and Restorative Dentistry, São Paulo State University (UNESP), School of Dentistry, Araçatuba, Rua José Bonifácio 1193, Araçatuba, SP, Cep 16015-050, Brazil.
Department of Materials Engineering, Federal University of São Carlos - UFSCar, São Carlos, SP, Cep 13565-905, Brazil.
Clin Oral Investig. 2021 May;25(5):2811-2820. doi: 10.1007/s00784-020-03597-7. Epub 2020 Sep 30.
To evaluate the effect of treatment with fluoridated toothpaste supplemented with a combination of sodium trimetaphosphate (TMP) and casein phosphopeptide-amorphous calcium phosphate (MI Paste Plus) on the demineralization of dental enamel.
Bovine enamel blocks selected by initial surface hardness (SHi) were randomly allocated into six groups (n = 12), according to the test toothpastes: (1) without F-TMP-MI Paste Plus (Placebo); (2) 1100 ppm F (1100F); (3) MI Paste Plus; (4) 1100F + MI Paste Plus (1100F-MI Paste Plus), (5) 1100F + 3 % TMP (1100F-TMP); and (6) 1100F-TMP + MI Paste Plus (1100F-TMP-MI Paste Plus). Blocks were treated two times per day with slurries of toothpaste (1 min), and groups 4 and 6 received an application of MI Paste Plus (3 min). Next, the samples were subjected to five pH cycles (demineralizing/remineralizing solutions) at 37 °C, to produce subsurface enamel lesions.Thereafter, the blocks were maintained for 2 days in fresh remineralizing solution. After pH cycling, the following were obtained: percentage of surface hardness loss (%SH); integrated loss of subsurface hardness (ΔKHN); profile analysis and lesion depth subsurface through polarized light microscopy (PLM); scanning electron microscopy (SEM); and fluoride (F), calcium (Ca), and phosphorus (P) in the enamel. The data were subjected to ANOVA (1-criterion), followed by the Student-Newman-Keuls test (p < 0.001).
The 1100F-TMP-MI Paste Plus group showed better results for SHR, ΔKHN, and PLM (p < 0.001). The F concentration was similar among all groups (p > 0.001). The 1100F-TMP-MI Paste Plus group showed the highest concentration of Ca and P in the enamel (p < 0.001).
The application of 1100F-TMP-MI Paste Plus promoted a higher inhibitory effect against enamel demineralization.
The combination of treatments with F, TMP, and MI Paste Plus can be an effective alternative to improve the oral health of individuals, especially those with high activity of dental caries and at high risk for its development.
评估含氟牙膏联合三聚磷酸钠（TMP）和酪蛋白磷酸肽-无定形磷酸钙（MI Paste Plus）治疗对牙釉质脱矿的影响。
根据测试牙膏，将初始表面硬度（SHi）选择的牛牙釉质块随机分为六组（n=12）：（1）不含 F-TMP-MI Paste Plus（安慰剂）；（2）1100 ppm F（1100F）；（3）MI Paste Plus；（4）1100F+MI Paste Plus（1100F-MI Paste Plus）；（5）1100F+3%TMP（1100F-TMP）；和（6）1100F-TMP+MI Paste Plus（1100F-TMP-MI Paste Plus）。块每天用牙膏糊（1 分钟）处理两次，第 4 组和第 6 组接受 MI Paste Plus（3 分钟）的应用。然后，将样本在 37°C 下进行五次 pH 循环（脱矿/再矿化溶液），以产生表面下牙釉质损伤。此后，将块在新鲜再矿化溶液中保持 2 天。pH 循环后，获得以下结果：表面硬度损失百分比（%SH）；表面下硬度综合损失（ΔKHN）；偏振光显微镜下的表面下硬度轮廓分析和损伤深度（PLM）；扫描电子显微镜（SEM）；以及牙釉质中的氟（F）、钙（Ca）和磷（P）。数据采用单因素方差分析（1 准则），然后采用学生-纽曼-凯斯检验（p<0.001）。
1100F-TMP-MI Paste Plus 组的 SHR、ΔKHN 和 PLM 结果更好（p<0.001）。所有组之间的 F 浓度相似（p>0.001）。1100F-TMP-MI Paste Plus 组牙釉质中 Ca 和 P 的浓度最高（p<0.001）。
应用 1100F-TMP-MI Paste Plus 可对牙釉质脱矿产生更高的抑制作用。
F、TMP 和 MI Paste Plus 的联合治疗可以作为改善个体口腔健康的有效方法，尤其是对那些具有高龋齿活动度和高龋齿发展风险的个体。
