Qi Ge, Hua Hong, Gao Yan, Lin Qin, Yu Guang-yan
Department of Oral and Maxillofacial Surgery Peking University School and Hospital of Stomatology, Beijing, China.
Chin Med J (Engl). 2009 Mar 5;122(5):556-60.
Sjögren syndrome (SS) is a systematic autoimmune disease, on which traditional therapeutic agents show limited effect. More effective agents with longer-lasting and fewer side effects are needed in the clinic. The aim of this study was to investigate the effects of Ganoderma lucindum spores (GLS) on sialoadenitis of nonobese diabetic (NOD) mice.
Thirty-two female NOD mice were assigned randomly into 4 groups: low-dose GLS-treated (L-GLS) group and high-dose GLS-treated (H-GLS) group, a dexamethasone group, and a normal saline (NS) control group. Stimulated total saliva flow rate (STFR), area of lymphocytic infiltration in submandibular glands and ratios of CD4(+) and CD8(+) T lymphocytes and B lymphocytes in peripheral blood as well as apoptosis of these subsets and serum IgG level were tested after 10 weeks of treatments. Differences among the groups were analyzed by one-way analysis of variance (ANOVA), Student-Newman-Keuls Test (SNK) was used between each two groups and a P < 0.05 was considered statistically significant.
STFR of the high-dose GLS group increased significantly after a 10-week treatment compared with those of the NS control group (P < 0.05). The incidence of sialoadenitis in GLS-treated NOD mice groups showed no significant difference compared with the control group (P > 0.05), but the area of lymphocytic foci in both the H-GLS and L-GLS groups decreased significantly to 50% of the NS control group (P < 0.05); the ratio of CD4(+)/CD8(+) T lymphocytes and apoptosis of B lymphocytes of NOD mice with sialoadenitis were less and apoptosis of CD4(+) and CD8(+) T lymphocytes were significantly increased compared with the control group (P < 0.05). After pretreatment with H-GLS before sialoadenitis onset, the ratio of CD4(+)/CD8(+) T lymphocyte and the serum IgG levels of NOD mice decreased significantly (P < 0.05).
Pretreatment with H-GLS can relieve symptoms of sialoadenitis in NOD mice. GLS has some protective effects on sialoadenitis in NOD mice through increasing STFR and decreasing the area of lymphocytic foci by regulating the ratio of CD4(+)/CD8(+) T and apoptosis of B lymphocytes.
干燥综合征（SS）是一种系统性自身免疫性疾病，传统治疗药物对其疗效有限。临床上需要更有效、作用持久且副作用更少的药物。本研究旨在探讨灵芝孢子（GLS）对非肥胖糖尿病（NOD）小鼠涎腺炎的影响。
将32只雌性NOD小鼠随机分为4组：低剂量GLS治疗组（L-GLS组）、高剂量GLS治疗组（H-GLS组）、地塞米松组和生理盐水（NS）对照组。治疗10周后，检测刺激后总唾液流速（STFR）、下颌下腺淋巴细胞浸润面积、外周血CD4(+)和CD8(+) T淋巴细胞及B淋巴细胞比例，以及这些亚群的凋亡情况和血清IgG水平。组间差异采用单因素方差分析（ANOVA），两组间比较采用Student-Newman-Keuls检验（SNK），P < 0.05认为差异有统计学意义。
高剂量GLS组治疗10周后STFR较NS对照组显著升高（P < 0.05）。GLS治疗的NOD小鼠组涎腺炎发病率与对照组相比无显著差异（P > 0.05），但H-GLS组和L-GLS组淋巴细胞灶面积均显著降低至NS对照组的50%（P < 0.
