灵芝孢子对2型糖尿病大鼠附睾细胞线粒体钙离子及细胞色素C的影响

[Effects of ganoderma lucidum spores on mitochondrial calcium ion and cytochrome C in epididymal cells of type 2 diabetes rats].

作者信息

Ma Xiao-ru, Zhou Cheng-fu, Wang Shu-qiu, Wang Wei-qun, Liu Yue-xia, Wang Shu-xiang, Wang Fang-fang, Zhang Jian-hua, Li Yong-yi

机构信息

Department of Pathophysiology, Basic Medical Science College, Jiamusi University, Jiamusi, Heilongjiang 154007, China.

出版信息

Zhonghua Nan Ke Xue. 2007 May;13(5):400-2.

PMID:17569252

Abstract

OBJECTIVE

To observe the effects of ganoderma lucidum spores (GLS) on mitochondrial calcium ion and cytochrome C in the epididymal cells of type 2 diabetes rats.

METHODS

Fifty adolescent rats were randomly divided into a model group (n=20), a GLS group (n=20) and a control group (n=10). The animals of the former two groups were injected with 2% STZ via vena caudalis for one time to induce type 2 diabetes. Then the model group was given high-fat-sugar diet, the GLS group high-fat-sugar diet + GLS (250 mg/kg x d), and the control group normal diet + CA-citrate sodium buffer. The bilateral epididymides were obtained 10 weeks later and the contents of mitochondrial calcium and cytochrome C detected.

RESULTS

Type 2 diabetes models were successfully constructed. The content of mitochondrial calcium in the epididymal cells was significantly higher in the model group ([3.279 +/- 0.502] mg/L) than in the control group ([2.606 +/- 0.048] mg/L, P < 0.01), with no significant difference between the GLS group ([2.693 +/- 0. 196] mg/L) and the control (P > 0.05). In the model group, the content of mitochondrial cytochrome C ([3.213 +/- 1.511] micromol/L) was significantly lower (P < 0.05) while that of cytoplasm cytochrome C ([2.484 +/- 0.661] micromol/L) significantly higher (P < 0.05) than in the control ([5.688 +/- 1.679] micromol/L and [1.574 +/- 0.329] micromol/L, respectively). In the GLS group, the content of mitochondrial cytochrome C ([5.258 +/- 1.560] micromol/L) was higher, with no significant difference (P > 0.05), and that of cytoplasm cytochrome C ([1.727 +/- 0.396] micromol/L) significantly lower than in the model group (P < 0.05), but the difference between the GLS and the control group was not significant (P > 0.05).

CONCLUSION

With disequilibrium of calcium homeostasis and damage to mitochondria, there might be excessive apoptosis in the epididymal cells of type 2 diabetes rats. Ganoderma lucidum spores could protect epididymal cells and counteract their apoptosis in diabetic condition.

摘要

目的

观察灵芝孢子粉（GLS）对2型糖尿病大鼠附睾细胞线粒体钙离子及细胞色素C的影响。

方法

将50只青春期大鼠随机分为模型组（n = 20）、GLS组（n = 20）和对照组（n = 10）。前两组动物经尾静脉一次性注射2%链脲佐菌素诱导2型糖尿病。然后模型组给予高脂高糖饮食，GLS组给予高脂高糖饮食+GLS（250mg/kg×d），对照组给予正常饮食+枸橼酸钠缓冲液。10周后取双侧附睾，检测线粒体钙和细胞色素C含量。

结果

成功构建2型糖尿病模型。模型组附睾细胞线粒体钙含量（[3.279±0.502]mg/L）显著高于对照组（[2.606±0.048]mg/L，P<0.01），GLS组（[2.693±0.196]mg/L）与对照组比较差异无统计学意义（P>0.05）。模型组线粒体细胞色素C含量（[3.213±1.511]μmol/L）显著低于对照组（[5.688±1.679]μmol/L，P<0.05），而胞质细胞色素C含量（[2.484±0.661]μmol/L）显著高于对照组（[1.574±0.329]μmol/L，P<0.05）。GLS组线粒体细胞色素C含量（[5.258±1.560]μmol/L）较高，与对照组比较差异无统计学意义（P>0.05），胞质细胞色素C含量（[1.727±0.396]μmol/L）显著低于模型组（P<0.05），但与对照组比较差异无统计学意义（P>0.05）。