Lyons D J, Mitchell E B, Mitchell D N
Division of Immunological Medicine, MRC Clinical Research Center, Harrow, UK.
Biomed Pharmacother. 1991;45(4-5):187-92. doi: 10.1016/0753-3322(91)90106-4.
Granuloma formation in patients with sarcoidosis may be evoked by the intradermal injection of homogenised sarcoid tissue (the Kveim reaction). Attempts to demonstrate an in vitro counterpart of the reaction have been unsuccessful. The cytokine interleukin-2 (IL-2) enhances immune responses in vivo and in vitro. We report here an attempt to amplify the Kveim reaction by the addition of IL-2. We studied the effect of Kveim reagent on the proliferative responses of peripheral blood mononuclear cells (PBMC) in the presence or absence of exogenous IL-2. Twenty-eight patients were studied and 14 healthy subjects served as controls. PBMC were cultured, in vitro, in the presence of Kveim reagent. Recombinant IL-2 or both of these combined. Proliferative responses were measured by [3H]-thymidine incorporation. The response of patients' PBMC in the presence of Kveim reagent at a dilution of 1:40 was significantly below the unstimulated response (P less than 0.01). Kveim reagent at a dilution of 1:40 also inhibited the proliferative response of patients PBMC to IL-2 (P less than 0.005); greater dilutions (1:100 and 1:1000) of Kveim reagent were not inhibitory. Responses of PBMC from control subjects (both unstimulated and IL-2 generated) were reduced in the presence of Kveim reagent, however, these reductions were not statistically significant.
结节病患者的肉芽肿形成可通过皮内注射均质化的结节病组织诱发（克维姆反应）。试图证明该反应的体外对应物的尝试均未成功。细胞因子白细胞介素-2（IL-2）在体内和体外均可增强免疫反应。我们在此报告了一项通过添加IL-2来增强克维姆反应的尝试。我们研究了在存在或不存在外源性IL-2的情况下，克维姆试剂对外周血单核细胞（PBMC）增殖反应的影响。研究了28名患者，14名健康受试者作为对照。PBMC在体外与克维姆试剂一起培养，或与重组IL-2或两者一起培养。通过[3H] - 胸腺嘧啶核苷掺入法测量增殖反应。在1:40稀释度的克维姆试剂存在下，患者PBMC的反应显著低于未刺激的反应（P小于0.01）。1:40稀释度的克维姆试剂也抑制了患者PBMC对IL-2的增殖反应（P小于0.005）；更高稀释度（1:100和1:1000）的克维姆试剂没有抑制作用。然而，在克维姆试剂存在下，对照受试者PBMC的反应（未刺激和IL-2诱导的）均降低，但这些降低没有统计学意义。
