Department of Animal Sciences, University of Illinois at Urbana - Champaign, Urbana, IL.
Evonik Nutrition & Care GmbH, Hanau-Wolfgang, Germany.
J Anim Sci. 2019 Oct 3;97(10):4227-4234. doi: 10.1093/jas/skz275.
An experiment was conducted to test the hypothesis that Val from a spray dried L-Val fermentation biomass (Val-FB; 64.4% L-Val) has a bioavailability of 100% relative to Val from L-Val (98% L-Val) when fed to weanling pigs. A Val-deficient basal diet containing 0.63% standardized ileal digestible (SID) Val was formulated. Six additional diets were prepared by supplementing the basal diet with 0.08%, 0.17%, or 0.25% L-Val or 0.12%, 0.25%, or 0.37% Val-FB to create experimental diets from both Val sources that contained 0.71%, 0.79%, or 0.87% SID Val. Two hundred twenty-four weaned pigs (6.87 ± 0.64 kg initial BW) were allotted to a randomized complete block design with 7 diets, 4 pigs per pen, and 8 replicate pens per diet. Diets were fed for 20 d. At the conclusion of the experiment, a blood sample from 1 pig per pen was analyzed for blood urea nitrogen (BUN) and plasma free AA. A linear regression model was used to estimate the relative bioavailability (RBV) of Val in Val-FB relative to Val from L-Val. Results indicated that the final BW and ADG were greater (P < 0.01) for pigs fed diets supplemented with Val-FB than pigs fed diets supplemented with L-Val. The ADFI decreased (linear, P ≤ 0.01), whereas G:F increased (linear, P < 0.01) by increasing inclusion of both Val sources in the diets. Regardless of source of dietary Val, BUN values were reduced (linear and quadratic, P < 0.01) as the concentration of Val in the diet increased. Pigs fed diets supplemented with L-Val had increased (linear and quadratic, P ≤ 0.05) concentrations of Val and Arg in plasma, and plasma concentrations of Ile, Leu, Lys, Ala, Cys, and Pro linearly increased (P < 0.05). There was also an increase (linear, P < 0.05) in plasma concentrations of Ile, Leu, Met, Ala, Asp, Cys, and Pro as Val-FB was added to the diets, and the concentration of Val in plasma increased (linear and quadratic, P < 0.05). Using L-Val as the standard, the RBV of Val in Val-FB as determined by ADG, G:F, and final BW was 146%, 135%, and 143%, respectively, with 95% confidence intervals of 99% to 191%, 83% to 187%, and 70% to 217%, respectively. In conclusion, the linear regression estimated a RBV of at least 100% for Val in Val-FB relative to Val from L-Val, and pigs fed diet supplemented with Val-FB had greater final BW, ADG, and G:F than pigs fed diets supplemented with the same amount of Val from L-Val.
进行了一项实验，以检验以下假设：来自喷雾干燥 L-缬氨酸发酵生物质（Val-FB；64.4% L-缬氨酸）的 Val 的生物利用度相对于 L-缬氨酸（98% L-缬氨酸）为 100%，当喂给断奶仔猪时。配制了一种含有 0.63%标准化回肠可消化（SID）Val 的 Val 缺乏基础日粮。通过在基础日粮中添加 0.08%、0.17%或 0.25% L-Val 或 0.12%、0.25%或 0.37% Val-FB，从两种 Val 来源制备了另外 6 种补充日粮，以创建含有 0.71%、0.79%或 0.87% SID Val 的实验日粮。224 头断奶仔猪（初始 BW 为 6.87±0.64kg）被分配到一个随机完全块设计中，有 7 种日粮，每栏 4 头猪，每种日粮 8 个重复栏。日粮喂养 20 天。实验结束时，从每栏的 1 头猪中抽取血样进行血液尿素氮（BUN）和血浆游离氨基酸（Free AA）分析。使用线性回归模型来估计 Val-FB 中 Val 的相对生物利用度（RBV）相对于 L-Val 中 Val 的相对生物利用度。结果表明，与饲喂 L-Val 补充日粮的仔猪相比，饲喂 Val-FB 补充日粮的仔猪最终 BW 和 ADG 更高（P<0.01）。ADFI 降低（线性，P≤0.01），而 G:F 增加（线性，P<0.01），因为两种 Val 来源在日粮中的添加量增加。无论 Val 的饮食来源如何，随着日粮中 Val 浓度的增加，BUN 值均降低（线性和二次，P<0.01）。饲喂 L-Val 补充日粮的仔猪 Val 和 Arg 在血浆中的浓度增加（线性和二次，P≤0.05），血浆中 Ile、Leu、Lys、Ala、Cys 和 Pro 的浓度线性增加（P<0.05）。随着 Val-FB 添加到日粮中，血浆中 Ile、Leu、Met、Ala、Asp、Cys 和 Pro 的浓度也增加（线性，P<0.05），并且血浆中 Val 的浓度增加（线性和二次，P<0.05）。使用 L-Val 作为标准，ADG、G:F 和最终 BW 测定的 Val-FB 中 Val 的 RBV 分别为 146%、135%和 143%，95%置信区间分别为 99%至 191%、83%至 187%和 70%至 217%。总之，线性回归估计 Val-FB 中 Val 的 RBV 至少为 100%，相对于 L-Val 中 Val，饲喂补充 Val-FB 的日粮的仔猪的最终 BW、ADG 和 G:F 高于饲喂相同量 L-Val 的仔猪。
