髓过氧化物酶作为牙周疾病的炎症标志物：大鼠实验研究

Myeloperoxidase as inflammatory marker of periodontal disease: experimental study in rats.

作者信息

Gomes Debora A S, Pires Juliana R, Zuza Elizangela P, Muscara Marcelo N, Herrera Bruno S, Spolidorio Luís C, Toledo Benedicto E C, Spolidorio Denise M P

机构信息

Department of Diagnosis and Oral Surgery, State University of Sao Paulo, Araraquara, Sao Paulo, Brazil.

出版信息

Immunol Invest. 2009;38(2):117-22. doi: 10.1080/08820130802457503.

DOI:10.1080/08820130802457503

PMID:19330621

Abstract

RATIONALE

Previous studies have used myeloperoxidase (MPO) as an inflammatory marker to estimate the accumulation of neutrophils in inflamed regions.

OBJECTIVE

The aim of this experimental study was to quantify the levels of MPO related to experimental periodontal disease in rats.

METHODS

Periodontal disease was induced in a group of rats using placement of a ligature around molar teeth. A group of rats without ligature placement served as a control. Measurements were made on the 3(rd), 7(th), 15(th) and 30(th) day from baseline. Gingival tissues were taken for quantification of MPO levels by ELISA.

RESULTS

The rats with induced periodontal disease showed statistically higher MPO levels (p < 0.05) when compared to control rats. A significant increase in the levels of MPO released on days 7 and 30 was observed, with higher levels in the group with induced periodontitis.

CONCLUSION

The levels of MPO were found to be higher in rats with induced periodontal disease, confirming the hypothesis that MPO may serve as an inflammatory marker for periodontitis.

摘要