Ukaegbu Kelechi, Foyle Deborah, Luan Xianghong, Schneiderman Emet, Allen Edward P, Plemons Jacqueline, Svoboda Kathy K H
Department of Periodontology, Texas A&M School of Dentistry, Dallas, Texas, USA.
Department of Biomedical Sciences, Texas A&M School of Dentistry, Dallas, Texas, USA.
J Periodontol. 2024 Nov;95(11):1086-1096. doi: 10.1002/JPER.23-0794. Epub 2024 Jun 3.
Prolonged inflammation and oxidative stress can impede healing. To enhance healing efficiency, many solutions have been employed. This is an in vivo study comparing chlorhexidine (CHX) to a commercial antioxidant gel (AO).
Envelope flaps were created in the lower incisor gingival region of 60 Sprague-Dawley rats, and acellular dermal matrix (ADM) was inserted. Animals were randomly assigned to postsurgical treatment application of AO gel or 0.12% CHX twice daily. A control group received no postsurgical treatment. Data collected (before surgery, 24 h, and 72 h) included surgical images, tissue samples, and weights. Blinded scorers assessed images using a wound healing scale. Real-time polymerase chain reaction (RT-PCR) was used for gene expression of tumor necrosis factor-alpha (TNFα), interleukin-1 (IL-1), myeloperoxidase (MPO), and superoxide dismutase (SOD).
The AO group scored higher than the CHX and control groups in clinical evaluation (p < 0.05). At 24 h, TNFα expression was upregulated in the AO group compared to CHX (p = 0.027) and controls (p = 0.018). The AO group had significantly higher expression of antioxidant enzyme (SOD) at 24 h compared to CHX (p = 0.021). All animals lost weight in the first 24 h. Animals treated with AO or CHX regained more weight at 72 h than control animals (p = 0.034 and 0.003, respectively).
Animals treated with AO healed faster. AO led to earlier upregulation of TNFα and antioxidant enzyme SOD. We hypothesized that AO promoted an earlier inflammatory process while counteracting oxidative stress by increasing antioxidant responses via SOD.
长期炎症和氧化应激会阻碍愈合。为提高愈合效率，人们采用了多种方法。这是一项在活体动物身上进行的研究，比较洗必泰（CHX）与一种商业抗氧化凝胶（AO）的效果。
在60只Sprague-Dawley大鼠的下切牙牙龈区域制作信封状皮瓣，并植入脱细胞真皮基质（ADM）。动物被随机分配接受术后每天两次涂抹AO凝胶或0.12% CHX的治疗。对照组不接受术后治疗。收集的数据（手术前、24小时和72小时）包括手术图像、组织样本和体重。不知情的评分者使用伤口愈合量表评估图像。采用实时聚合酶链反应（RT-PCR）检测肿瘤坏死因子-α（TNFα）、白细胞介素-1（IL-1）、髓过氧化物酶（MPO）和超氧化物歧化酶（SOD）的基因表达。
在临床评估中AO组得分高于CHX组和对照组（p < 0.05）。在24小时时，与CHX组（p = 0.027）和对照组（p = 0.018）相比，AO组中TNFα表达上调。与CHX组相比，AO组在24小时时抗氧化酶（SOD）的表达显著更高（p = 0.021）。所有动物在最初24小时内体重均减轻。接受AO或CHX治疗的动物在72小时时比对照动物体重恢复得更多（分别为p = 0.034和0.003）。
接受AO治疗的动物愈合更快。AO导致TNFα和抗氧化酶SOD更早上调。我们推测AO通过SOD增加抗氧化反应来促进更早的炎症过程，同时对抗氧化应激。
