Pittsburgh NMR Center for Biomedical Research and Department of Biological Sciences, Carnegie Mellon University, Pittsburgh, Pennsylvania, USA.
Nanomedicine. 2012 Nov;8(8):1345-54. doi: 10.1016/j.nano.2012.02.017. Epub 2012 Mar 7.
Non-invasive in vivo tracking of T-cells by magnetic resonance imaging (MRI) can lead to a better understanding of many pathophysiological situations, including AIDS, cancer, diabetes, graft rejection. However, an efficient MRI contrast agent and a reliable technique to track non-phagocytic T-cells are needed. We report a novel superparamagnetic nano-sized iron-oxide particle, IOPC-NH2 series particles, coated with polyethylene glycol (PEG), with high transverse relaxivity (250 s(-1) mM(-1)), thus useful for MRI studies. IOPC-NH2 particles are the first reported magnetic particles that can label rat and human T-cells with over 90% efficiency, without using transfection agents, HIV-1 transactivator peptide, or electroporation. IOPC-NH2 particles do not cause any measurable effects on T-cell properties. Infiltration of IOPC-NH2-labeled T-cells can be detected in a rat model of heart-lung transplantation by in vivo MRI. IOPC-NH2 is potentially valuable contrast agents for labeling a variety of cells for basic and clinical cellular MRI studies, e.g., cellular therapy.
In this study, a novel PEG coated superparamagnetic nano-sized iron-oxide particle was investigated as a T-cell labeling agent for MRI studies. The reported particles can label T-cells with over 90% efficiency, without using transfection agents, HIV-1 transactivator peptide, or electroporation, therefore may enable more convenient preclinical call labeling studies.
通过磁共振成像（MRI）对 T 细胞进行非侵入性体内跟踪，可以更好地了解许多病理生理情况，包括艾滋病、癌症、糖尿病、移植物排斥。然而，需要一种有效的 MRI 对比剂和一种可靠的技术来跟踪非吞噬性 T 细胞。我们报告了一种新型超顺磁纳米氧化铁粒子 IOPC-NH2 系列粒子，表面涂有聚乙二醇（PEG），具有高横向弛豫率（250 s(-1) mM(-1)），因此可用于 MRI 研究。IOPC-NH2 粒子是第一批报道的可将大鼠和人 T 细胞标记效率超过 90%的磁性粒子，而无需使用转染试剂、HIV-1 转录激活肽或电穿孔。IOPC-NH2 粒子对 T 细胞特性没有任何可测量的影响。在心肺移植大鼠模型中，通过体内 MRI 可以检测到 IOPC-NH2 标记 T 细胞的浸润。IOPC-NH2 是一种很有前途的对比剂，可用于标记各种细胞，用于基础和临床细胞 MRI 研究，例如细胞治疗。
在这项研究中，研究人员研究了一种新型的 PEG 包裹的超顺磁纳米氧化铁粒子，作为 MRI 研究中的 T 细胞标记剂。报告的粒子可以以超过 90%的效率标记 T 细胞，而无需使用转染试剂、HIV-1 转录激活肽或电穿孔，因此可能使更方便的临床前细胞标记研究成为可能。
