Xu-Dong Shen, Zan Shen, Shui-er Zheng, Li-na Tang, Wen-xi Yu, Feng Lin, Yang Yao
Department of Oncology, Shanghai 6th People's Hospital, Shanghai Jiao Tong University, Shanghai, China.
Clin Invest Med. 2009 Apr 1;32(2):E180-8. doi: 10.25011/cim.v32i2.6036.
To determine the prognostic value of the expression of Ezrin, CD44 and Six1 genes in osteosarcoma tissues of Chinese patients.
Fluorescent quantitative real-time PCR was applied to study the mRNA levels of Ezrin, CD44 and Six1 genes in 32 osteosarcoma patient samples and 10 adjacent normal tissues and MG63 osteosarcoma cell lines. The analysis of relationships between pulmonary metastasis and overall survival time were carried out based on the clinical data.
mRNA levels of Ezrin and Six1 genes in osteosarcoma tissues were higher than those in adjacent normal tissues (P=0.015, 0.025). The mRNA levels of Ezrin, CD44 and Six1 genes were closely correlated with Enneking GTM clinical staging, while no correlations were demonstrated between the mRNA level of these genes with sex, age, location or pathological types. In addition, we demonstrated that the high mRNA level of Ezrin gene was related to shorter lung metastasis-free and overall survival time of the Chinese patients with osteosarcoma (P < 0.001).
Our data suggest that Ezrin, but not CD44 and Six1, could be a prognostic factor and a predictor of potential lung metastasis in osteosarcoma. Further large sample studies need to be done to confirm the potential value of Ezrin as a new therapeutic target.
确定Ezrin、CD44和Six1基因在中国骨肉瘤患者组织中的表达的预后价值。
应用荧光定量实时PCR研究32例骨肉瘤患者样本、10例相邻正常组织及MG63骨肉瘤细胞系中Ezrin、CD44和Six1基因的mRNA水平。基于临床数据对肺转移与总生存时间之间的关系进行分析。
骨肉瘤组织中Ezrin和Six1基因的mRNA水平高于相邻正常组织（P = 0.015，0.025）。Ezrin、CD44和Six1基因的mRNA水平与Enneking GTM临床分期密切相关，而这些基因的mRNA水平与性别、年龄、部位或病理类型之间未显示相关性。此外，我们证明Ezrin基因的高mRNA水平与中国骨肉瘤患者较短的无肺转移生存期和总生存期相关（P < 0.001）。
我们的数据表明，Ezrin而非CD44和Six1可能是骨肉瘤的一个预后因素和潜在肺转移的预测指标。需要进一步开展大样本研究以证实Ezrin作为新治疗靶点的潜在价值。
