圆二色光谱法在蛋白质二级结构分析方面存在固有局限性。
Circular dichroism spectroscopy has intrinsic limitations for protein secondary structure analysis.
作者信息
Khrapunov Sergei
机构信息
Department of Biochemistry, Albert Einstein College of Medicine, 1300 Morris Park Avenue, Bronx, NY 10461, USA.
出版信息
Anal Biochem. 2009 Jun 15;389(2):174-6. doi: 10.1016/j.ab.2009.03.036. Epub 2009 Mar 28.
Abstract
Secondary structure content (SSC) cannot be calculated accurately from circular dichroism (CD) spectra for the majority of proteins whose three-dimensional structures have been solved. "Reliable" SSC that is significantly different from random SSC can be calculated from CD spectra only for all-alpha proteins and all-beta proteins with canonical beta-strand geometry.
摘要
对于大多数三维结构已得到解析的蛋白质而言，无法从圆二色性（CD）光谱准确计算二级结构含量（SSC）。只有对于具有典型β链几何结构的全α蛋白和全β蛋白，才能从CD光谱计算出与随机SSC有显著差异的“可靠”SSC。
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