Greenfield Norma J
Department of Neuroscience and Cell Biology, Robert Wood Johnson Medical School, 675 Hoes Lane West, Piscataway, New Jersey 08854-8021, USA.
Nat Protoc. 2006;1(6):2733-41. doi: 10.1038/nprot.2006.229.
Circular dichroism (CD) is an excellent tool for examining the interactions and stability of proteins. This protocol covers methods to obtain and analyze circular dichroism spectra to measure changes in the folding of proteins as a function of denaturants, osmolytes or ligands. Applications include determination of the free energy of folding of a protein, the effects of mutations on protein stability and the estimation of binding constants for the interactions of proteins with other proteins, DNA or ligands, such as substrates or inhibitors. The experiments require 2-5 h.
圆二色性（CD）是研究蛋白质相互作用和稳定性的一种出色工具。本方案涵盖了获取和分析圆二色光谱的方法，以测量蛋白质折叠随变性剂、渗透压调节剂或配体的变化。应用包括测定蛋白质折叠的自由能、突变对蛋白质稳定性的影响以及估算蛋白质与其他蛋白质、DNA或配体（如底物或抑制剂）相互作用的结合常数。这些实验需要2至5小时。
