McCarthy M M, Coirini H, Schumacher M, Pfaff D W, McEwen B S, Schwartz-Giblin S
Rockefeller University, New York, NY 10021.
Brain Res. 1991 Aug 16;556(2):321-3. doi: 10.1016/0006-8993(91)90323-n.
[3H]Muscimol binding was measured in the lumbar spinal cord of female rats by in vitro quantitative autoradiography. Ovariectomized rats were treated subcutaneously with either oil, estradiol benzoate (EB) or EB plus progesterone (P) in a regime known to reliably induce sexual receptivity. The level of [3H]muscimol binding was highest in laminae I-III and in the region around the central canal. Binding was lower in laminae IV-VI and was frequently undetectable in the ventral horn. There was a significant increase in the level of binding in laminae I-III after EB treatment. There was also a significant increase after treatment with EB+P in comparison to both the ovariectomized and EB-treated groups in this same region of the spinal cord.
通过体外定量放射自显影法测定雌性大鼠腰段脊髓中[³H]蝇蕈醇结合情况。对去卵巢大鼠皮下注射油、苯甲酸雌二醇（EB）或EB加孕酮（P），采用已知能可靠诱导性接受能力的给药方案。[³H]蝇蕈醇结合水平在Ⅰ-Ⅲ层和中央管周围区域最高。在Ⅳ-Ⅵ层结合水平较低，在腹角通常检测不到。EB处理后，Ⅰ-Ⅲ层结合水平显著升高。与去卵巢组和EB处理组相比，在脊髓的同一区域，EB + P处理后结合水平也显著升高。
