Schwartz-Giblin S, Canonaco M, McEwen B S, Pfaff D W
Laboratory of Neurobiology and Behavior, Rockefeller University, New York, NY 10021.
Neuroscience. 1988 Apr;25(1):249-57. doi: 10.1016/0306-4522(88)90023-1.
Tritiated flunitrazepam binding was measured in autoradiograms of lumbar spinal cord from control ovariectomized female rats and from ovariectomized females treated with either estradiol-silastic implants or one subcutaneous injection of 500 micrograms progesterone 4-5 h before being killed or both estradiol and progesterone. Four areas of spinal cord were analyzed: the substantia gelatinosa, the region leading to and around the central canal, the remainder of the dorsal horn and the ventral horn. Specific flunitrazepam binding was greatest in the substantia gelatinosa and in the region around the central canal and was weakest in the ventral horn. Estradiol had no effect on flunitrazepam binding in the spinal cord despite estradiol concentrating cells in the substantia gelatinosa and around the central canal. In vivo progesterone enhanced flunitrazepam binding in the substantia gelatinosa while 10(-8) M progesterone in vitro was without effect. One day after spinal transection flunitrazepam binding in endocrine controls and estradiol-treated animals was unchanged but a progesterone injection 4-5 h before being killed had no effect on binding in the substantia gelatinosa.
在对照去卵巢雌性大鼠以及在处死前4 - 5小时接受雌二醇硅橡胶植入物或皮下注射500微克孕酮或同时接受雌二醇和孕酮治疗的去卵巢雌性大鼠的腰脊髓放射自显影片中，测定了氚标记氟硝西泮的结合情况。分析了脊髓的四个区域：胶状质、中央管及其周围区域、背角其余部分和腹角。氟硝西泮的特异性结合在胶状质和中央管周围区域最大，在腹角最弱。尽管雌二醇在胶状质和中央管周围区域聚集细胞，但它对脊髓中氟硝西泮的结合没有影响。体内孕酮增强了胶状质中氟硝西泮的结合，而体外10(-8) M孕酮则没有作用。脊髓横断一天后，内分泌对照动物和接受雌二醇治疗的动物中氟硝西泮的结合没有变化，但在处死前4 - 5小时注射孕酮对胶状质中的结合没有影响。
