再生丝素蛋白膜的细胞相容性：一种适用于创伤愈合的医用生物材料。
Cytocompatibility of regenerated silk fibroin film: a medical biomaterial applicable to wound healing.
机构信息
Cell and Molecular Biology Institute, College of Medicine, Soochow University, Suzhou 215123, China.
出版信息
J Zhejiang Univ Sci B. 2010 Jan;11(1):10-6. doi: 10.1631/jzus.B0900163.
OBJECTIVE
To explore the feasibility of using regenerated silk fibroin membrane to construct artificial skin substitutes for wound healing, it is necessary to evaluate its cytocompatibility.
METHODS
The effects of regenerated silk fibroin film on cytotoxicity, adhesion, cell cycle, and apoptosis of L929 cells, growth and vascular endothelial growth factor (VEGF) expression of ECV304 cells, and VEGF, angiopoietin-1 (Ang-1), platelet-derived growth factor (PDGF) and fibroblast growth factor 2 (FGF2) expression of WI-38 cells were assessed by 3-(4,5)-dimethylthiahiazo (-z-y1)-3,5-di-phenytetrazoliumromide (MTT) assay, viable cell counting, flow cytometry (FCM), and enzyme-linked immunosorbant assay (ELISA).
RESULTS
We showed that the regenerated silk fibroin film was not cytotoxic to L929 cells and had no adverse influence on their adhesion, cell cycle or apoptosis; it had no adverse influence on the growth and VEGF secretion of ECV304 cells and no effect on the secretion of VEGF, Ang-1, PDGF and FGF2 by WI-38 cells.
CONCLUSION
The regenerated silk fibroin film should be an excellent biomaterial with good cytocompatibility, providing a framework for reparation after trauma in clinical applications.
目的
为了探索利用再生丝素膜构建用于创伤愈合的人工皮肤替代物的可行性，有必要评估其细胞相容性。
方法
通过 3-（4,5）-二甲基噻唑（-z-y1）-3,5-二苯基四唑溴盐（MTT）测定、活细胞计数、流式细胞术（FCM）和酶联免疫吸附试验（ELISA）评估再生丝素膜对 L929 细胞的细胞毒性、黏附、细胞周期和细胞凋亡的影响，以及对 ECV304 细胞的血管内皮生长因子（VEGF）表达和 WI-38 细胞的 VEGF、血管生成素-1（Ang-1）、血小板衍生生长因子（PDGF）和碱性成纤维细胞生长因子 2（FGF2）表达的影响。
结果
我们表明，再生丝素膜对 L929 细胞无细胞毒性，对其黏附、细胞周期或凋亡无不良影响；对 ECV304 细胞的生长和 VEGF 分泌无不良影响，对 WI-38 细胞的 VEGF、Ang-1、PDGF 和 FGF2 分泌也无影响。
结论
再生丝素膜应该是一种具有良好细胞相容性的优秀生物材料，为临床应用中的创伤后修复提供了框架。
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