Shibuya Kenichi, Yamada Tetsuya, Ichimura Kazuo
National Institute of Floricultural Science, National Agriculture and Food Research Organization,2-1 Fujimoto, Tsukuba, Japan.
Autophagy. 2009 May;5(4):546-7. doi: 10.4161/auto.5.4.8310. Epub 2009 May 27.
Petal senescence is a type of programmed cell death (PCD) that is tightly regulated by multiple genes. We recently reported that a putative membrane protein, InPSR26, regulates progression of PCD during petal senescence in Japanese morning glory (Ipomoea nil). Reduced InPSR26 expression in transgenic plants (PSR26r lines) resulted in accelerated petal senescence with hastened development of PCD symptoms, and transcript levels of autophagy-related genes were reduced in the petals. Autophagy visualized by monodansylcadaverine staining indicated reduced autophagic activity in the PSR26r plants. The results from our recent studies suggest that InPSR26 acts to delay the progression of PCD during petal senescence, possibly through regulation of the autophagic process. In this addendum, we discuss the role of autophagy in petal senescence as it relates to these findings.
花瓣衰老属于一种程序性细胞死亡（PCD），受到多个基因的严格调控。我们最近报道，一种假定的膜蛋白InPSR26在日本牵牛花（圆叶牵牛）花瓣衰老过程中调节PCD进程。转基因植物（PSR26r系）中InPSR26表达降低导致花瓣衰老加速，PCD症状发展加快，花瓣中自噬相关基因的转录水平降低。单丹磺酰尸胺染色显示的自噬表明PSR26r植物中的自噬活性降低。我们最近的研究结果表明，InPSR26可能通过调节自噬过程来延缓花瓣衰老过程中PCD的进程。在本附录中，我们讨论了自噬在花瓣衰老中的作用及其与这些发现的关系。
