University of Medicine and Pharmacy Iuliu Haţieganu, Clinical Emergency Hospital Professor Dr. Octavian Fodor, Cluj-Napoca, Romania.
J Gastrointestin Liver Dis. 2009 Mar;18(1):45-50.
To investigate the HCV genotype distribution in Romania in the first national study, to establish the correlations with epidemiological, biochemical, virological and histological features and to compare our results with those from neighboring countries.
Two distinct groups of patients and two methods were used: 153 patients in the frame of ACHIEVE study with genotyping and subtypes determination (Versant HCV genotype 2.0 assay) and 461 patients in the frame of an Epidemiological National Multicenter Study having only genotype determination with a commercial kit (Roche Molecular System). Epidemiological, biochemical, virological and histological features were investigated only in the ENMS group.
Genotype 1b was found in 93.46% (ACHIEVE study) and genotype 1 (without subtype identification) in 99.13% of patients (ENMS study). Percutaneous routes of transmission were found in 85.9% of cases. The prevalence of HCV infection increased with age. A high viral load (> or = 600,000 IU/ml) was found in 67.9% of patients, especially those older than 40 years. Significant fibrosis > or = F2 was present in patients older than 40 years (70.9%). There were no correlations between HCV-RNA levels and histological features or between ALT levels and METAVIR activity or fibrosis scores. A similar homogeneity of HCV genotype distribution has been reported for Moldavia (96%) and Hungary (94.5%).
Type 1 HCV genotype was found almost exclusively in Romanian patients with chronic hepatitis C by two different methods of investigation. The pattern showed by this distribution in Romania and some neighboring countries suggests an epidemic profile of HCV infection.
在首次全国性研究中调查罗马尼亚的 HCV 基因型分布，确定与流行病学、生化、病毒学和组织学特征的相关性，并将我们的结果与邻国进行比较。
使用两种不同的方法和两组患者：ACHIEVE 研究中的 153 名患者进行基因分型和亚型确定（Versant HCV 基因型 2.0 检测），以及在一项流行病学全国多中心研究中，仅使用商业试剂盒进行基因型确定的 461 名患者（罗氏分子系统）。仅在 ENMS 组中研究了流行病学、生化、病毒学和组织学特征。
ACHIEVE 研究中发现基因型 1b 占 93.46%，ENMS 研究中发现基因型 1（无亚型鉴定）占 99.13%。85.9%的病例为经皮传播途径。HCV 感染的患病率随年龄增长而增加。67.9%的患者病毒载量较高（≥600,000 IU/ml），尤其是年龄大于 40 岁的患者。年龄大于 40 岁的患者存在显著纤维化（≥F2）（70.9%）。HCV-RNA 水平与组织学特征之间、ALT 水平与 METAVIR 活动或纤维化评分之间均无相关性。摩尔达维亚（96%）和匈牙利（94.5%）的 HCV 基因型分布也存在类似的均一性。
通过两种不同的调查方法，在罗马尼亚的慢性丙型肝炎患者中几乎仅发现 1 型 HCV 基因型。这种分布模式表明，罗马尼亚和一些邻国的 HCV 感染呈流行特征。
