Li Hongtao, Wang Lili, Ye Lu, Mao Yan, Xie Xuhua, Xia Chao, Chen Jia, Lu Zimin, Song Jianxin
Department of Infectious Diseases, Tongji Hospital, Wuhan, China.
Med Microbiol Immunol. 2009 May;198(2):113-21. doi: 10.1007/s00430-009-0111-z. Epub 2009 Apr 1.
Quorum sensing system is a cell-to-cell communication system that plays a pivotal role in virulence expression in bacteria. Recent advances have demonstrated that the Pseudomonas aeruginosa quorum sensing molecule, N-3-oxododecanoyl homoserine lactone (3OC(12)-HSL), exerts effects on mammalian cells and modulates host immune response. Mast cells (MCs) are strategically located in the tissues that are constantly exposed to external stimulus. Therefore, it is very much possible that 3OC(12)-HSL may interact with MCs. Little is known, however, about specific effects of 3OC(12)-HSL on MCs. To address this, we investigated the influence of 3OC(12)-HSL on cell viability, apoptosis, intracellular calcium and cytokine release in MCs. We found that at high concentrations (100 microM), 3OC(12)-HSL inhibited proliferation and induced apoptosis in P815. The 3OC(12)-HSL treatment significantly increased intracellular calcium release in both P815 and HMC-1. We also observed that 3OC(12)-HSL-induced histamine release and degranulation in HMC-1 cells. Furthermore, 3OC(12)-HSL-induced IL-6 production at lower concentrations (6.25-12.5 microM) but steadily reduced IL-6 production at high concentration (50-100 muM). These data demonstrate that P. aeruginosa 3OC(12)-HSL affects MCs function.
群体感应系统是一种细胞间通讯系统，在细菌的毒力表达中起关键作用。最近的研究进展表明，铜绿假单胞菌群体感应分子N-3-氧代十二烷酰高丝氨酸内酯（3OC(12)-HSL）对哺乳动物细胞有影响并调节宿主免疫反应。肥大细胞（MCs）战略性地分布在不断暴露于外部刺激的组织中。因此，3OC(12)-HSL很有可能与MCs相互作用。然而，关于3OC(12)-HSL对MCs的具体影响知之甚少。为了解决这个问题，我们研究了3OC(12)-HSL对MCs细胞活力、凋亡、细胞内钙和细胞因子释放的影响。我们发现，在高浓度（100 microM）下，3OC(12)-HSL抑制P815细胞增殖并诱导其凋亡。3OC(12)-HSL处理显著增加了P815和HMC-1细胞内的钙释放。我们还观察到3OC(12)-HSL诱导HMC-1细胞组胺释放和脱颗粒。此外，3OC(12)-HSL在较低浓度（6.25-12.5 microM）时诱导IL-6产生，但在高浓度（50-100 microM）时稳定降低IL-6产生。这些数据表明铜绿假单胞菌的3OC(12)-HSL影响MCs功能。
