Kassis Amira N, Kubow Stan, Jones Peter J H
School of Dietetics and Human Nutrition, McGill University, Montreal, QC, Canada.
Lipids. 2009 May;44(5):391-6. doi: 10.1007/s11745-009-3295-5. Epub 2009 Apr 1.
Sugar cane policosanols (SCP) have been shown to exert antioxidant properties in various studies conducted in Cuba. Independent studies have since reported no significant effect of SCP consumption on oxidized LDL levels. The objective of the present study was to confirm the effects of Cuban SCP on LDL oxidation using a high-precision capture ELISA procedure in hypercholesterolemic individuals. Twenty-one otherwise healthy hypercholesterolemic men and post-menopausal women participated in a randomized double blind crossover study where they received 10 mg/day of policosanol or a placebo incorporated in margarine as an evening snack for a period of 28 days. Subjects maintained their usual dietary and exercise habits throughout the duration of the study. Blood was collected on the first as well as the last 2 days of the trial. LDL oxidation was measured from plasma using a solid phase two-site enzyme immunoassay. A lack of effect of SCP was observed on LDL cholesterol levels, as well as no difference in LDL oxidation between the SCP treatment and placebo at the end of the intervention period. Subject body weights remained stable throughout the study and showed no significant correlation with LDL oxidation levels. Absolute levels of plasma LDL cholesterol were significantly (P < 0.05) correlated with plasma concentrations of oxidized LDL. The findings of the present study suggest that SCP do not significantly affect LDL oxidation. Our results align with results of recent policosanol research questioning the efficacy of these natural extracts as cardio-protective agents.
在古巴开展的多项研究表明，甘蔗多廿醇（SCP）具有抗氧化特性。此后，独立研究报告称，食用SCP对氧化型低密度脂蛋白（LDL）水平无显著影响。本研究的目的是，采用高精度捕获酶联免疫吸附测定法，在高胆固醇血症个体中确认古巴SCP对LDL氧化的影响。21名其他方面健康的高胆固醇血症男性和绝经后女性参与了一项随机双盲交叉研究，他们连续28天每天摄入10毫克多廿醇或添加在人造黄油中的安慰剂作为晚间小吃。在整个研究期间，受试者保持其通常的饮食和运动习惯。在试验的第一天以及最后两天采集血液。使用固相双位点酶免疫测定法测定血浆中的LDL氧化情况。观察到SCP对LDL胆固醇水平没有影响，在干预期结束时，SCP治疗组和安慰剂组之间的LDL氧化也没有差异。在整个研究过程中，受试者体重保持稳定，且与LDL氧化水平无显著相关性。血浆LDL胆固醇的绝对水平与氧化型LDL的血浆浓度显著相关（P < 0.05）。本研究结果表明，SCP不会显著影响LDL氧化。我们的结果与近期多廿醇研究的结果一致，这些研究对这些天然提取物作为心脏保护剂的功效提出了质疑。
