小鼠微小病毒非结构基因中存在的大内含子的剪接受位于非共识供体下游的TIA-1/TIAR结合调控。
Splicing of the large intron present in the nonstructural gene of minute virus of mice is governed by TIA-1/TIAR binding downstream of the nonconsensus donor.
作者信息
Choi Eun-Young, Pintel David
机构信息
Department of Molecular Microbiology and Immunology, University of Missouri-Columbia School of Medicine, Life Sciences Center, Columbia, Missouri 65211, USA.
出版信息
J Virol. 2009 Jun;83(12):6306-11. doi: 10.1128/JVI.00213-09. Epub 2009 Apr 1.
Abstract
The essential proteins NS1 and NS2 of minute virus of mice are encoded by mRNAs R1 and R2, respectively. R2 is derived from R1 by excision of a large intron and thus splicing governs the relative ratios of NS1 and NS2. Excision of the large intron utilizes a nonconsensus 5' donor site. We identified a U-rich and A-rich intronic sequence immediately downstream of the nonconsensus 5' donor site that functions as an intronic splicing enhancer (ISE) required for efficient large-intron excision. The ISE binds the cellular RNA-processing proteins TIA-1 and TIAR, which enhance usage of the nonconsensus donor.
摘要
小鼠微小病毒的必需蛋白NS1和NS2分别由mRNA R1和R2编码。R2是通过切除一个大的内含子从R1衍生而来的，因此剪接决定了NS1和NS2的相对比例。大内含子的切除利用了一个非共识性的5'供体位点。我们在非共识性5'供体位点下游立即鉴定出一个富含U和富含A的内含子序列，该序列作为有效切除大内含子所需的内含子剪接增强子（ISE）发挥作用。ISE结合细胞RNA加工蛋白TIA-1和TIAR，这两种蛋白增强了非共识供体的使用。
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