使用MGIT 960和EpiCenter仪器对结核分枝杆菌进行药物敏感性定量检测。
Quantitative drug susceptibility testing of Mycobacterium tuberculosis by use of MGIT 960 and EpiCenter instrumentation.
作者信息
Springer Burkhard, Lucke Katja, Calligaris-Maibach Romana, Ritter Claudia, Böttger Erik C
机构信息
Institut für Medizinische Mikrobiologie, Universität Zürich, Gloriastrasse 30/32, CH-8006 Zürich, Switzerland.
出版信息
J Clin Microbiol. 2009 Jun;47(6):1773-80. doi: 10.1128/JCM.02501-08. Epub 2009 Apr 1.
Abstract
Since numbers of drug-resistant Mycobacterium tuberculosis strains are on the rise, the simple classification into "susceptible" and "resistant" strains based on susceptibility testing at "critical concentrations" has to be reconsidered. While future studies have to address the correlation of phenotypic resistance levels and treatment outcomes, a prerequisite for corresponding investigations is the ability to exactly determine levels of quantitative drug resistance in clinical M. tuberculosis isolates. Here we have established the conditions for quantitative drug susceptibility testing for first- and second-line agents using MGIT 960 instrumentation and EpiCenter software equipped with the TB eXiST module. In-depth comparative analysis of a range of well-characterized susceptible and resistant clinical isolates has allowed us to propose conditions for testing and to develop criteria for interpretation.
摘要
由于耐药结核分枝杆菌菌株的数量在不断增加，基于“临界浓度”药敏试验将菌株简单分类为“敏感”和“耐药”的方法必须重新考虑。虽然未来的研究需要解决表型耐药水平与治疗结果之间的相关性，但相应研究的一个先决条件是能够准确测定临床结核分枝杆菌分离株的定量耐药水平。在此，我们利用配备了TB eXiST模块的MGIT 960仪器和EpiCenter软件，建立了一线和二线药物定量药敏试验的条件。对一系列特征明确的敏感和耐药临床分离株进行深入的比较分析，使我们能够提出检测条件并制定解释标准。
相似文献
1
Quantitative drug susceptibility testing of Mycobacterium tuberculosis by use of MGIT 960 and EpiCenter instrumentation.使用MGIT 960和EpiCenter仪器对结核分枝杆菌进行药物敏感性定量检测。
J Clin Microbiol. 2009 Jun;47(6):1773-80. doi: 10.1128/JCM.02501-08. Epub 2009 Apr 1.
2
Molecular Screening Versus Phenotypic Susceptibility Testing of Multidrug-Resistant Mycobacterium tuberculosis Isolates for Streptomycin and Ethambutol.耐多药结核分枝杆菌分离株对链霉素和乙胺丁醇的分子筛查与表型药敏试验
Microb Drug Resist. 2018 Sep;24(7):923-931. doi: 10.1089/mdr.2017.0294. Epub 2018 Jan 16.
3
Comparison of line probe assay to BACTEC MGIT 960 system for susceptibility testing of first and second-line anti-tuberculosis drugs in a referral laboratory in South Africa.在南非一家转诊实验室中，用于一线和二线抗结核药物药敏试验的线性探针分析与BACTEC MGIT 960系统的比较。
BMC Infect Dis. 2017 Dec 28;17(1):795. doi: 10.1186/s12879-017-2898-3.
4
Comparison and development of pyrazinamide susceptibility testing methods for tuberculosis in Thailand.泰国结核分枝杆菌吡嗪酰胺药敏试验方法的比较与发展
Diagn Microbiol Infect Dis. 2015 Nov;83(3):270-3. doi: 10.1016/j.diagmicrobio.2015.07.008. Epub 2015 Jul 17.
5
Evaluation of the BACTEC MGIT 960 system and the resazurin microtiter assay for susceptibility testing of Mycobacterium tuberculosis to second-line drugs.评估BACTEC MGIT 960系统和刃天青微量滴定法用于结核分枝杆菌对二线药物药敏试验的情况。
J Microbiol Methods. 2017 Aug;139:168-171. doi: 10.1016/j.mimet.2017.06.007. Epub 2017 Jun 7.
6
Drug susceptibility testing of Mycobacterium tuberculosis against second-line drugs using the Bactec MGIT 960 System.使用Bactec MGIT 960系统对结核分枝杆菌进行二线药物药敏试验。
Int J Tuberc Lung Dis. 2008 Dec;12(12):1449-55.
7
Mycobacterial growth indicator tube for susceptibility testing of Mycobacterium tuberculosis to isoniazid and rifampin.用于结核分枝杆菌对异烟肼和利福平药敏试验的分枝杆菌生长指示管
Diagn Microbiol Infect Dis. 1997 Jul;28(3):153-6. doi: 10.1016/s0732-8893(97)00006-0.
8
Evaluation of whole genome sequencing and software tools for drug susceptibility testing of Mycobacterium tuberculosis.评估全基因组测序和软件工具在结核分枝杆菌药物敏感性检测中的应用。
Clin Microbiol Infect. 2019 Jan;25(1):82-86. doi: 10.1016/j.cmi.2018.03.041. Epub 2018 Apr 11.
9
Multicenter evaluation of fully automated BACTEC Mycobacteria Growth Indicator Tube 960 system for susceptibility testing of Mycobacterium tuberculosis.全自动BACTEC分枝杆菌生长指示管960系统用于结核分枝杆菌药敏试验的多中心评估
J Clin Microbiol. 2002 Jan;40(1):150-4. doi: 10.1128/JCM.40.1.150-154.2002.
10
Validation of bedaquiline drug-susceptibility testing by BACTEC MGIT 960 system for .采用BACTEC MGIT 960系统对贝达喹啉药物敏感性检测进行验证，用于…… （原文此处不完整）
Int J Mycobacteriol. 2019 Oct-Dec;8(4):329-332. doi: 10.4103/ijmy.ijmy_151_19.
引用本文的文献
1
Unpacking bedaquiline heteroresistance: the importance of intermediate profiles for phenotypic drug susceptibility testing.剖析贝达喹啉异质性耐药：中间型表型对药物敏感性检测的重要性。
Antimicrob Agents Chemother. 2025 Aug 6;69(8):e0035625. doi: 10.1128/aac.00356-25. Epub 2025 Jul 21.
2
Drug resistance profile of Mycobacterium tuberculosis complex isolated from pulmonary tuberculosis patients and their household contacts in central Ethiopia.从埃塞俄比亚中部肺结核患者及其家庭接触者中分离出的结核分枝杆菌复合群的耐药谱
BMC Infect Dis. 2025 Jun 20;25(1):806. doi: 10.1186/s12879-025-11220-x.
3
Unpacking bedaquiline hetero-resistance: the importance of intermediate profiles for phenotypic drug-susceptibility testing.剖析贝达喹啉异质性耐药：中间型表型对药物敏感性检测的重要性。
bioRxiv. 2025 Mar 10:2025.03.09.642295. doi: 10.1101/2025.03.09.642295.
4
Drug Exposure and Susceptibility of Pyrazinamide Correlate with Treatment Response in Pyrazinamide-Susceptible Patients with Multidrug-Resistant Tuberculosis.吡嗪酰胺的药物暴露量与易感性和耐多药结核病且对吡嗪酰胺敏感患者的治疗反应相关。
Pharmaceutics. 2024 Jan 21;16(1):144. doi: 10.3390/pharmaceutics16010144.
5
Prevalence of mutations associated with isoniazid and rifampicin resistance: A systematic review and -analysis.与异烟肼和利福平耐药相关的突变的流行情况：一项系统评价与分析
J Clin Tuberc Other Mycobact Dis. 2023 Jun 1;32:100379. doi: 10.1016/j.jctube.2023.100379. eCollection 2023 Aug.
6
Population pharmacokinetics and dose evaluations of linezolid in the treatment of multidrug-resistant tuberculosis.利奈唑胺治疗耐多药结核病的群体药代动力学及剂量评估
Front Pharmacol. 2023 Jan 9;13:1032674. doi: 10.3389/fphar.2022.1032674. eCollection 2022.
7
Drug sensitivity of clinical isolates of and its association with bacterial genotype in the Somali region, Eastern Ethiopia.和在埃塞俄比亚东部索马里地区的临床分离株的药敏与其细菌基因型的相关性。
Front Public Health. 2022 Aug 17;10:942618. doi: 10.3389/fpubh.2022.942618. eCollection 2022.
8
Utility of line probe assay in detecting drug resistance and the associated mutations in patients with extrapulmonary tuberculosis in Addis Ababa, Ethiopia.线性探针分析在埃塞俄比亚亚的斯亚贝巴肺外结核患者中检测耐药性及相关突变的效用
SAGE Open Med. 2022 May 20;10:20503121221098241. doi: 10.1177/20503121221098241. eCollection 2022.
9
Evaluation of Isoniazid Derivatives as Potential Agents Against Drug-Resistant Tuberculosis.异烟肼衍生物作为抗耐药结核病潜在药物的评估
Front Pharmacol. 2022 May 4;13:868545. doi: 10.3389/fphar.2022.868545. eCollection 2022.
10
Correlation of Mutations with the Minimum Inhibitory Concentrations of Fluoroquinolones among Multidrug-Resistant Isolates in Bangladesh.孟加拉国耐多药分离株中氟喹诺酮类药物最低抑菌浓度与突变的相关性
Pathogens. 2021 Nov 2;10(11):1422. doi: 10.3390/pathogens10111422.
本文引用的文献
1
Tuberculosis drug resistance in an area of low endemicity in 2004 to 2006: semiquantitative drug susceptibility testing and genotyping.2004年至2006年低流行地区的结核病耐药性：半定量药物敏感性检测和基因分型
J Clin Microbiol. 2008 Dec;46(12):4064-7. doi: 10.1128/JCM.01114-08. Epub 2008 Oct 15.
2
Tuberculosis: drug resistance, fitness, and strategies for global control.结核病：耐药性、适应性及全球控制策略
Eur J Pediatr. 2008 Feb;167(2):141-8. doi: 10.1007/s00431-007-0606-9. Epub 2007 Nov 7.
3
Genotype MTBDRplus: a further step toward rapid identification of drug-resistant Mycobacterium tuberculosis.基因型MTBDRplus：迈向快速鉴定耐药结核分枝杆菌的又一步。
J Clin Microbiol. 2008 Jan;46(1):393-4. doi: 10.1128/JCM.01066-07. Epub 2007 Oct 31.
4
Worldwide emergence of extensively drug-resistant tuberculosis.广泛耐药结核病在全球范围内的出现。
Emerg Infect Dis. 2007 Mar;13(3):380-7. doi: 10.3201/eid1303.061400.
5
Evaluation of the GenoType MTBDRplus assay for rifampin and isoniazid susceptibility testing of Mycobacterium tuberculosis strains and clinical specimens.用于结核分枝杆菌菌株和临床标本利福平及异烟肼药敏试验的GenoType MTBDRplus检测方法的评估
J Clin Microbiol. 2007 Aug;45(8):2635-40. doi: 10.1128/JCM.00521-07. Epub 2007 May 30.
6
From magic bullets back to the magic mountain: the rise of extensively drug-resistant tuberculosis.从神奇子弹回到魔山：广泛耐药结核病的兴起
Nat Med. 2007 Mar;13(3):295-8. doi: 10.1038/nm0307-295.
7
Emergence of Mycobacterium tuberculosis with extensive resistance to second-line drugs--worldwide, 2000-2004.2000 - 2004年全球范围内对二线药物具有广泛耐药性的结核分枝杆菌的出现
MMWR Morb Mortal Wkly Rep. 2006 Mar 24;55(11):301-5.
8
Evaluation of MGIT 960-based antimicrobial testing and determination of critical concentrations of first- and second-line antimicrobial drugs with drug-resistant clinical strains of Mycobacterium tuberculosis.基于MGIT 960的抗微生物药物敏感性检测以及耐多药临床结核分枝杆菌一线和二线抗微生物药物临界浓度的测定
J Clin Microbiol. 2006 Mar;44(3):811-8. doi: 10.1128/JCM.44.3.811-818.2006.
9
Multicenter laboratory validation of the BACTEC MGIT 960 technique for testing susceptibilities of Mycobacterium tuberculosis to classical second-line drugs and newer antimicrobials.BACTEC MGIT 960技术用于检测结核分枝杆菌对经典二线药物和新型抗菌药物敏感性的多中心实验室验证
J Clin Microbiol. 2006 Mar;44(3):688-92. doi: 10.1128/JCM.44.3.688-692.2006.
10
Ethambutol resistance testing by mutation detection.通过突变检测进行乙胺丁醇耐药性检测。
Int J Tuberc Lung Dis. 2006 Jan;10(1):68-73.
Zotero 插件