Kikkawa Yayoi S, Nakagawa Takayuki, Horie Rie T, Ito Juichi
Department of Otolaryngology-Head and Neck Surgery, Graduate School of Medicine, Kyoto University, Kyoto, Japan.
Neuroreport. 2009 May 6;20(7):689-94. doi: 10.1097/WNR.0b013e32832a5c68.
Reactive oxygen species (ROS) play a role in the degeneration of auditory hair cells because of aging, noise trauma, or ototoxic drugs. Hydrogenation is a fundamental reduction/de-oxidation reaction in living organisms. This study thus examined the potential of hydrogen to protect auditory hair cells from ROS-induced damage. To generate ROS, we applied antimycin A to explant cultures of auditory epithelia, and examined the effect of hydrogen on the protection of hair cells against ROS. Incubation with a hydrogen-saturated medium significantly reduced ROS generation and subsequent lipid peroxidation in the auditory epithelia, leading to increased survival of the hair cells. These findings show the potential of hydrogen to protect auditory hair cells from ROS-induced damage.
活性氧（ROS）在因衰老、噪声损伤或耳毒性药物导致的听觉毛细胞退化中起作用。氢化是生物体中的一种基本还原/脱氧反应。因此，本研究考察了氢气保护听觉毛细胞免受ROS诱导损伤的潜力。为了产生ROS，我们将抗霉素A应用于听觉上皮的外植体培养物，并研究了氢气对保护毛细胞免受ROS损伤的作用。用氢气饱和培养基孵育可显著减少听觉上皮中的ROS生成及随后的脂质过氧化，从而提高毛细胞的存活率。这些发现表明氢气具有保护听觉毛细胞免受ROS诱导损伤的潜力。
