Jochová J, Rupes I, Streiblová E
Institute of Microbiology, Czechoslovak Academy of Sciences, Prague.
Cell Biol Int Rep. 1991 Jul;15(7):607-10. doi: 10.1016/0309-1651(91)90007-6.
By rhodamine-phalloidin fluorescence, distinct continuous F-actin rings were visualized in 18-20% of the protoplasts of Schizosaccharomyces pombe and S. japonicus var. versatilis, in addition to randomly distributed F-actin dots. Whereas the reversion of ring-lacking protoplasts coincided with the polarization of the dotted F-actin pattern, the ring-containing protoplasts became furrowed as the F-actin rings constricted. The furrowing was more conspicuous in S. japonicus var. versatilis than in S. pombe protoplasts and it was blocked when the reversion was inhibited by Novozyme 234 indicating that the cell wall formation is essential for the F-actin ring constriction.
通过罗丹明-鬼笔环肽荧光观察发现，在粟酒裂殖酵母和粟酒裂殖酵母变种多形酵母18%-20%的原生质体中，除了随机分布的F-肌动蛋白点外，还可见明显连续的F-肌动蛋白环。缺乏环的原生质体的回复与点状F-肌动蛋白模式的极化同时发生，而含有环的原生质体随着F-肌动蛋白环的收缩而出现沟纹。在粟酒裂殖酵母变种多形酵母中，沟纹比在粟酒裂殖酵母原生质体中更明显，当用诺维信234抑制回复时，沟纹被阻断，这表明细胞壁形成对于F-肌动蛋白环的收缩至关重要。
