New Zealand's National School of Pharmacy, University of Otago, Dunedin, New Zealand.
J Pharm Sci. 2009 Nov;98(11):4191-204. doi: 10.1002/jps.21724.
Swelling and phase behaviour of phytantriol and glyceryl monooleate (GMO) matrices with varying water loadings were investigated. Release of a model protein, FITC-Ova was subsequently examined. Polarised light microscopy and small angle X-ray scattering analysis showed that the addition of FITC-Ova only altered the liquid crystalline structure of phytantriol matrices at low water loadings, and that postrelease study, the phase structure of matrices at both low and high loading reflected that of the binary system. Addition of FITC-Ova to GMO matrices also altered the liquid crystalline structure when compared to the respective binary system at low but not at high loading. All samples analysed after the release study had transformed to the reverse hexagonal phase (H(II)). Swelling studies revealed a faster and more extensive swelling of GMO when compared to phytantriol. Release of FITC-Ova from phytantriol matrices was faster and occurred to a greater extent most likely due to the conversion of GMO matrices into the H(II) phase. No effect on release as a function of initial water content was observed for either lipid. We have confirmed that phytantriol based liquid crystalline matrices can sustain the release of a hydrophilic protein, suggesting its suitability as a potential sustained antigen-delivery system.
研究了不同水载量下植物三醇和甘油单油酸酯（GMO）基质的肿胀和相行为。随后检查了模型蛋白 FITC-Ova 的释放情况。偏光显微镜和小角 X 射线散射分析表明，FITC-Ova 的添加仅在低水载量下改变了植物三醇基质的液晶结构，而在释放后研究中，低载量和高载量的基质的相结构均反映了二元系统的相结构。与相应的二元系统相比，FITC-Ova 添加到 GMO 基质中也会在低载量下改变液晶结构，但在高载量下不会。释放研究后分析的所有样品均已转变为反向六方相（H(II)）。与植物三醇相比，GMO 的溶胀研究显示出更快和更广泛的溶胀。FITC-Ova 从植物三醇基质中的释放更快，释放程度更大，这很可能是由于 GMO 基质转化为 H(II)相。两种脂质均未观察到初始水含量对释放的影响。我们已经证实，基于植物三醇的液晶基质可以维持亲水性蛋白的释放，这表明其适合作为潜在的持续抗原递送系统。
