Wang J J, Chang J P, Hsu C Y, Liang H M, Moller P C
Cell Tissue Res. 1983;233(2):367-76. doi: 10.1007/BF00238303.
Data from studies of ascitic cells of Chang hepatoma have shown that acid phosphatase (ACPase) can be localized simultaneously within the trans portion of the Golgi apparatus and in tubules of the Golgi-endoplasmic reticulum-lysosome (GERL) system. Reaction products of thiamine pyrophosphatase (TPPase) were also present consistently within trans elements of the Golgi apparatus and within GERL tubules. These new findings indicate that a close physiological association may exist between the Golgi apparatus and GERL, a concept that is consistent with previous observations of fibroblasts. When horseradish peroxidase (PO) is injected intraperitoneally into ascites-bearing rats and the ascitic cells withdrawn at different time intervals, PO could be localized within vesicles and tubules in the GERL region but could not be detected within the Golgi apparatus. Bulk-phase endocytosis requires a long time and a high concentration of PO to occur. The presence of PO within GERL indicates that this organelle may play a role in transporting or processing of certain exogenous proteins.
对张氏肝癌腹水细胞的研究数据表明，酸性磷酸酶（ACPase）可同时定位于高尔基体的反式部分以及高尔基体内质网溶酶体（GERL）系统的小管中。硫胺素焦磷酸酶（TPPase）的反应产物也始终存在于高尔基体的反式元件和GERL小管内。这些新发现表明，高尔基体与GERL之间可能存在密切的生理联系，这一概念与之前对成纤维细胞的观察结果一致。当将辣根过氧化物酶（PO）腹腔注射到腹水大鼠体内，并在不同时间间隔提取腹水细胞时，PO可定位于GERL区域的囊泡和小管内，但在高尔基体中未检测到。大容量内吞作用需要较长时间和高浓度的PO才能发生。GERL中PO的存在表明该细胞器可能在某些外源蛋白的运输或加工中起作用。
