Guo Jiaying, Luo Xiaoying, Wang Sen, He Lan, Zhao Junlong
State Key Laboratory of Agricultural Microbiology, College of Veterinary Medicine, Huazhong Agricultural University, Wuhan 430070, China.
Key Laboratory of Preventive Veterinary Medicine in Hubei Province, Wuhan 430070, China.
Vaccines (Basel). 2020 Oct 16;8(4):613. doi: 10.3390/vaccines8040613.
Human babesiosis caused by is an emerging threat for severe illness and even death, with an increasing impact worldwide. Currently, the regimen of atovaquone and azithromycin is considered as the standard therapy for treating human babesiosis, which, however, may result in drug resistance and relapse, suggesting the necessity of developing new drugs to control . In this regard, natural products are promising candidates for drug design against due to their active therapeutic efficacy, lower toxicity, and fewer adverse reactions to host. Here, the potential inhibitors against B. microti were preliminarily screened from 133 natural products, and 47 of them were selected for further screening. Gossypol (Gp) and xanthohumol (Xn) were finally shown to effectively inhibit the growth of with IC values of 8.47 μm and 21.40 μm, respectively. The cytotoxicity results showed that Gp and Xn were non-toxic to erythrocytes at a concentration below 100 μm. Furthermore, both of them were confirmed to be non-toxic to different types of cells in previous studies. Our findings suggest the potential of Gp and Xn as effective drugs against infection.
由[病原体名称未给出]引起的人类巴贝斯虫病是一种对严重疾病甚至死亡的新出现威胁，在全球范围内的影响日益增加。目前，阿托伐醌和阿奇霉素方案被认为是治疗人类巴贝斯虫病的标准疗法，然而，这可能会导致耐药性和复发，这表明开发新药来控制[病原体名称未给出]的必要性。在这方面，天然产物因其具有积极的治疗效果、较低的毒性以及对宿主较少的不良反应，是药物设计对抗[病原体名称未给出]的有希望的候选物。在此，从133种天然产物中初步筛选了针对微小巴贝斯虫的潜在抑制剂，其中47种被选用于进一步筛选。棉酚（Gp）和黄腐酚（Xn）最终显示能有效抑制[病原体名称未给出]的生长，IC值分别为8.47μm和21.40μm。细胞毒性结果表明，在浓度低于100μm时，Gp和Xn对红细胞无毒。此外，在先前的研究中证实它们对不同类型的细胞均无毒。我们的研究结果表明Gp和Xn作为对抗[病原体名称未给出]感染的有效药物的潜力。
