Minhaz Ud-Dean S M
Department of Genetic Engineering and Biotechnology, University of Dhaka, Dhaka, Bangladesh,
Syst Synth Biol. 2008 Dec;2(3-4):67-73. doi: 10.1007/s11693-009-9023-x. Epub 2009 Apr 3.
This theoretical scheme is intended to formulate a potential method for high fidelity synthesis of Nucleic Acid molecules towards a few thousand bases using an enzyme system. Terminal Deoxyribonucleotidyl Transferase, which adds a nucleotide to the 3'OH end of a Nucleic Acid molecule, may be used in combination with a controlled method for nucleotide addition and degradation, to synthesize a predefined Nucleic Acid sequence. A pH control system is suggested to regulate the sequential activity switching of different enzymes in the synthetic scheme. Current practice of synthetic biology is cumbersome, expensive and often error prone owing to the dependence on the ligation of short oligonucleotides to fabricate functional genetic parts. The projected scheme is likely to render synthetic genomics appreciably convenient and economic by providing longer DNA molecules to start with.
该理论方案旨在制定一种潜在方法，利用酶系统高保真合成数千个碱基的核酸分子。末端脱氧核苷酸转移酶可将核苷酸添加到核酸分子的3'OH末端，它可与核苷酸添加和降解的控制方法结合使用，以合成预定义的核酸序列。建议采用pH控制系统来调节合成方案中不同酶的顺序活性切换。由于依赖短寡核苷酸连接来构建功能性遗传元件，合成生物学的当前实践繁琐、昂贵且往往容易出错。通过提供更长的DNA分子作为起始材料，预计该方案可能会使合成基因组学变得明显方便和经济。
