Screening for disorders of pyruvate metabolism by measuring the ratio of the rates of lactate production and pyruvate decarboxylation in cultured skin fibroblasts.
作者信息
Kuroda Y, Kawakami I, Kobashi H, Naito E, Ito M, Saijo T, Yokota I, Takeda E
机构信息
Department of Pediatrics, School of Medicine, University of Tokushima, Japan.
出版信息
Clin Chim Acta. 1991 May 31;199(1):17-22. doi: 10.1016/0009-8981(91)90004-v.
PMID:1934498
Abstract
We assayed the rates of lactate production from [1-14C]pyruvate and decarboxylation of [1-14C]pyruvate in cultured skin fibroblasts from 8 patients with disorders of pyruvate metabolism and 16 control subjects. The disorders of pyruvate metabolism could be more readily detected by measuring the ratio between the rates of lactate production and pyruvate decarboxylation by cultured skin fibroblasts than by measuring either the rate in isolation.
摘要
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