Ghandilyan Artak, Ilk Nadine, Hanhart Corrie, Mbengue Malick, Barboza Luis, Schat Henk, Koornneef Maarten, El-Lithy Mohamed, Vreugdenhil Dick, Reymond Matthieu, Aarts Mark G M
Wageningen University, Arboretumlaan, The Netherlands.
J Exp Bot. 2009;60(5):1409-25. doi: 10.1093/jxb/erp084.
The regulation of mineral accumulation in plants is genetically complex, with several genetic loci involved in the control of one mineral and loci affecting the accumulation of different minerals. To investigate the role of growth medium and organ type on the genetics of mineral accumulation, two existing (LerxKond, LerxAn-1) and one new (LerxEri-1) Arabidopsis thaliana Recombinant Inbred Line populations were raised on soil and hydroponics as substrates. Seeds, roots, and/or rosettes were sampled for the determination of their Ca, Fe, K, Mg, Mn, P or Zn concentrations. For seeds only, the concentration of phytate (IP6), a strong chelator of seed minerals, was determined. Correlations between minerals/IP6, populations, growth conditions, and organs were determined and mineral/IP6 concentration data were used to identify quantitative trait loci (QTLs) for these traits. A striking difference was found between QTLs identified for soil-grown versus hydroponics-grown populations and between QTLs identified for different plant organs. Three common QTLs were identified for several populations, growth conditions, and organs, one of which corresponded to the ERECTA locus, variation of which has a strong effect on plant morphology.
植物中矿物质积累的调控在遗传上很复杂，有几个基因位点参与一种矿物质的控制，还有一些位点影响不同矿物质的积累。为了研究生长培养基和器官类型对矿物质积累遗传学的作用，以土壤和水培法为基质培育了两个现有的拟南芥重组自交系群体（LerxKond、LerxAn-1）和一个新的群体（LerxEri-1）。采集种子、根和/或莲座叶用于测定它们的钙、铁、钾、镁、锰、磷或锌浓度。仅对种子测定了植酸盐（IP6）的浓度，植酸盐是种子矿物质的强螯合剂。确定了矿物质/IP6、群体、生长条件和器官之间的相关性，并使用矿物质/IP6浓度数据来鉴定这些性状的数量性状位点（QTL）。在为土壤种植群体与水培种植群体鉴定的QTL之间以及为不同植物器官鉴定的QTL之间发现了显著差异。为几个群体、生长条件和器官鉴定出了三个常见的QTL，其中一个与ERECTA位点相对应，该位点的变异对植物形态有强烈影响。
