Golubeva Yelena, Rogers Keith
Histotechnology Laboratory, NCI-Frederick, SAIC-Frederick, Frederick, MD, USA.
Methods Mol Biol. 2009;511:3-60. doi: 10.1007/978-1-59745-447-6_1.
Histology, as a mean of tissue visualization on a cellular level, is a fundamental tool in the study of cancer. The need for simultaneous delivery of quality histological material for pathological evaluation and subsequent genomic and proteomic studies, however, requires modification of traditional practices to include rapid isolation and stabilization of target tissue to preserve molecular integrity. Informative molecular analysis depends on the integrity of target molecules (RNA, DNA, and proteins) in the tissue during and after its collection. A reliable systematic approach to routine and genomic/proteomic sample collection and preparation presented is supported by detailed protocols.
组织学作为在细胞水平上观察组织的一种手段，是癌症研究中的一项基本工具。然而，要同时提供高质量的组织学材料用于病理评估以及后续的基因组和蛋白质组研究，就需要对传统做法进行改进，包括快速分离和稳定目标组织以保持分子完整性。信息丰富的分子分析取决于组织在采集期间及之后目标分子（RNA、DNA和蛋白质）的完整性。本文介绍的一种用于常规及基因组/蛋白质组样本采集和制备的可靠系统方法得到了详细方案的支持。
