Golubeva Yelena, Symer David
Histotechnology Laboratory, SAIC, Frederick National Lab of Cancer Research, NCI, NIH, Frederick, MD, USA.
Methods Mol Biol. 2014;1092:297-356. doi: 10.1007/978-1-60327-292-6_20.
The need for large-scale collection of rodent embryos and individual embryonic tissues for genomic and proteomic studies requires modification of traditional practices of embryo necropsy. The sample intended for transcriptome study should be rapidly dissected and stabilized to preserve its molecular integrity. The retrieval of high-quality RNA, DNA, and proteins from the target tissue is crucial for informative molecular analysis (e.g., gene profiling on microarray platform). We present a reliable method of collection and preparation of rodent embryos for genomic studies supported by detailed protocols and RNA extraction results for different stages of mouse embryonic development.
为了进行基因组和蛋白质组研究而大规模收集啮齿动物胚胎及单个胚胎组织，需要对传统的胚胎尸检方法进行改进。用于转录组研究的样本应迅速解剖并稳定处理，以保持其分子完整性。从目标组织中获取高质量的RNA、DNA和蛋白质对于有意义的分子分析（如在微阵列平台上进行基因谱分析）至关重要。我们提供了一种可靠的啮齿动物胚胎收集和制备方法用于基因组研究，并辅以详细的实验方案以及针对小鼠胚胎发育不同阶段的RNA提取结果。
