Wang Aina, Yu Haifeng, Gao Xin, Li Xiaojie, Qiao Shiyan
State Key Laboratory of Animal Nutrition, China Agricultural University, No. 2 Yuanmingyuan West Road, Beijing, People's Republic of China.
Antonie Van Leeuwenhoek. 2009 Jun;96(1):89-98. doi: 10.1007/s10482-009-9339-2. Epub 2009 Apr 5.
The effect of feeding Lactobacillus fermentum I5007 on the immune system of weaned pigs with or without E. coli challenge was determined. Twenty-four weaned barrows (6.07 +/- 0.63 kg BW) were randomly assigned to one of four treatments (N = 6) in a factorial design experiment. The first two treatments consisted of healthy piglets with half of the pigs receiving no treatment while the other half was orally administered with L. fermentum I5007 (10(8) CFU/ml) at a daily dose of 20 ml. Pigs in the second two treatments were challenged on the first day with 20 ml of E. coli K88ac (10(8) CFU/ml). Half of these pigs were not treated while the remaining pigs were treated with 20 ml of L. fermentum I5007 (10(8) CFU/ml). Peripheral blood lymphocytes subsets were determined using flow cytometry. The intestinal mucosal immunity of the pigs was monitored by real time polymerase chain reaction. The cytokine content of the pig's serum was also analyzed. Oral administration of L. fermentum I5007 increased blood CD4(+) lymphocyte subset percentage as well as tumor necrosis factor-alpha and interferon-gamma expression in the ileum. Pigs challenged with E. coli had elevated jejunal tumor necrosis factor-alpha while interferon-gamma expression was increased throughout the small intestine. There was no difference in the concentration of the cytokines interleukin-2, interleukin-6, tumor necrosis factor-alpha and interferon-gamma in the serum. CD8(+) and CD4(+)/CD8(+) in peripheral blood were not affected by treatment. In conclusion, L. fermentum I5007 can enhance T cell differentiation and induce ileum cytokine expression suggesting that this probiotic strain could modulate immune function in piglets.
本研究旨在确定饲喂发酵乳杆菌I5007对有无大肠杆菌攻毒的断奶仔猪免疫系统的影响。在一项析因设计实验中，将24头断奶公猪（体重6.07±0.63千克）随机分为四个处理组（每组6头）。前两个处理组为健康仔猪，其中一半不做处理，另一半口服发酵乳杆菌I5007（10⁸CFU/ml），每日剂量为20毫升。后两个处理组的仔猪在第一天用20毫升大肠杆菌K88ac（10⁸CFU/ml）进行攻毒。其中一半不做处理，其余仔猪用20毫升发酵乳杆菌I5007（10⁸CFU/ml）进行处理。采用流式细胞术测定外周血淋巴细胞亚群。通过实时聚合酶链反应监测仔猪的肠道黏膜免疫。同时分析仔猪血清中的细胞因子含量。口服发酵乳杆菌I5007可增加血液中CD4⁺淋巴细胞亚群百分比以及回肠中肿瘤坏死因子-α和干扰素-γ的表达。大肠杆菌攻毒的仔猪空肠肿瘤坏死因子-α升高，而整个小肠中干扰素-γ的表达增加。血清中白细胞介素-2、白细胞介素-6、肿瘤坏死因子-α和干扰素-γ的浓度无差异。外周血中的CD8⁺和CD4⁺/CD8⁺不受处理影响。总之，发酵乳杆菌I5007可增强T细胞分化并诱导回肠细胞因子表达，表明该益生菌菌株可调节仔猪的免疫功能。
