Malinska Dominika, Kudin Alexei P, Debska-Vielhaber Grazyna, Vielhaber Stefan, Kunz Wolfram S
Division of Neurochemistry, Department of Epileptology and Life & Brain Center, University Bonn, Bonn, Germany.
Methods Enzymol. 2009;456:419-37. doi: 10.1016/S0076-6879(08)04423-6.
The production of reactive oxygen species (ROS) has been implicated for numerous pathologic alterations, including neurodegeneration and aging. They are formed to a considerable extent by mitochondria by single electron reduction of molecular oxygen by competent electron donors like flavoproteins and semiubiqunone species. In this chapter, we evaluate quantitative methods for the detection of hydrogen peroxide and superoxide production. Applying these methods we compared the ROS production of isolated mitochondria of mouse brain and skeletal muscle. We substantiated previous evidence that most mitochondrial ROS are produced at complexes I and III of the respiratory chain and that the contribution of individual complexes to ROS production is tissue dependent.
活性氧（ROS）的产生与多种病理改变有关，包括神经退行性变和衰老。它们在很大程度上由线粒体通过黄素蛋白和半醌类等有效电子供体对分子氧进行单电子还原而形成。在本章中，我们评估了检测过氧化氢和超氧阴离子产生的定量方法。应用这些方法，我们比较了小鼠脑和骨骼肌分离线粒体的ROS产生情况。我们证实了先前的证据，即大多数线粒体ROS是在呼吸链的复合体I和复合体III产生的，并且各个复合体对ROS产生的贡献是组织依赖性的。
