基于分裂适体和分裂蛋白的活细胞中mRNA转录本成像
imaging of mRNA transcripts based on split-aptamer and split protein in living cells.
作者信息
Peng Yan, Li Mengqi, Gong Fuyu, Liu Xiaofei, Xiong Jirou, Wang Keran
机构信息
The Affiliated First Hospital of Fuyang Normal University, Fuyang Normal University Fuyang Anhui 236037 P.R. China
Fuyang Women and Children's Hospital Fuyang Anhui 236037 P.R. China.
出版信息
RSC Adv. 2024 Mar 26;14(15):10146-10151. doi: 10.1039/d3ra08475b.
Messenger RNA (mRNA) is an essential component of cell development and growth. However, the detection of endogenous mRNA in living cells is currently limited. To address this issue, we have developed a novel strategy that comprises split-aptamer and split fluorescent protein dual-color, "turn-on" probes that specifically target mRNA with complementary sequences. Our split-aptamer and split-protein-initiated fluorescence complementation (sAPiFC) approach for live-cell imaging has demonstrated selectivity, stability, and capability for targeting various mRNAs.
信使核糖核酸（mRNA）是细胞发育和生长的重要组成部分。然而，目前在活细胞中检测内源性mRNA仍存在局限性。为了解决这一问题，我们开发了一种新策略，该策略包括分裂适体和分裂荧光蛋白双色“开启”探针，可通过互补序列特异性靶向mRNA。我们用于活细胞成像的分裂适体和分裂蛋白引发的荧光互补（sAPiFC）方法已证明其具有选择性、稳定性以及靶向各种mRNA的能力。
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