Institute of Neurosciences, The Fourth Military Medical University, Xi'an, China.
Spinal Cord. 2009 Dec;47(12):852-6. doi: 10.1038/sc.2009.30. Epub 2009 Apr 7.
To develop a method for the study of spinal cord injury (SCI) that can visualize the blood vessels and is compatible with hematoxylin and eosin (HE) staining and immunohistochemical techniques.
Visualization of the vascular changes is important for the study of SCI. The original ferric tannate method can stain the spinal cord vasculature to its terminals, but the diffuse tannate precipitates spoil the delicacy of the picture. More importantly, it is incompatible with HE staining and immunohistochemical techniques, which is crucial for the study of SCI. We thus aimed to develop a modified ferric tannate method that could meet the requirement for the study of SCI.
This study was carried out in China.
The original ferric tannate method involves a two-step procedure: intravascular perfusion of tannic acid, followed by soaking the tissue sections in a solution of ferric chloride. In the modified method both chemicals were delivered through perfusion.
In the original method, diffuse ferric tannate precipitates blurred the profile of the vessels. More importantly, it was incompatible with either HE or immunostaining methods. Our modified method stained the blood vessels with clean background and was compatible with both HE staining and immunohistochemical techniques.
The modified method is far superior to the original method and meets the requirement for the study of SCI.
开发一种可可视化血管的脊髓损伤 (SCI) 研究方法，该方法与苏木精和伊红 (HE) 染色以及免疫组织化学技术兼容。
可视化血管变化对于 SCI 的研究很重要。原有的铁单宁酸法可以将脊髓血管染色到末梢，但弥散的单宁酸沉淀会破坏图像的精细度。更重要的是，它与 HE 染色和免疫组织化学技术不兼容，这对于 SCI 的研究至关重要。因此，我们旨在开发一种改良的铁单宁酸法，以满足 SCI 研究的要求。
本研究在中国进行。
原铁单宁酸法包括两步程序：血管内灌注单宁酸，然后将组织切片浸泡在氯化铁溶液中。在改良方法中，两种化学物质都通过灌注来传递。
在原方法中，弥散的铁单宁酸沉淀使血管轮廓模糊。更重要的是，它与 HE 染色或免疫染色方法均不兼容。我们的改良方法对血管染色具有干净的背景，与 HE 染色和免疫组织化学技术均兼容。
改良方法优于原方法，满足 SCI 研究的要求。
