保留硬脑膜完整性并允许测量脑脊液压力和脊髓血流的脊髓损伤大鼠模型。
Rat model of spinal cord injury preserving dura mater integrity and allowing measurements of cerebrospinal fluid pressure and spinal cord blood flow.
机构信息
Equipe universitaire 3509 Paris VII-Paris XI-Paris XIII, Microcirculation, Bioénergétique, Inflammation et Insuffisance circulatoire aiguë, Paris Diderot-Paris VII University, Paris, France.
出版信息
Eur Spine J. 2013 Aug;22(8):1810-9. doi: 10.1007/s00586-013-2744-2. Epub 2013 Mar 19.
Abstract
PURPOSES
Cerebrospinal fluid (CSF) pressure elevation may worsen spinal cord ischaemia after spinal cord injury (SCI). We developed a rat model to investigate relationships between CSF pressure and spinal cord blood flow (SCBF).
METHODS
Male Wistar rats had SCI induced at Th10 (n = 7) or a sham operation (n = 10). SCBF was measured using laser-Doppler and CSF pressure via a sacral catheter. Dural integrity was assessed using subdural methylene-blue injection (n = 5) and myelography (n = 5).
RESULTS
The SCI group had significantly lower SCBF (p < 0.0001) and higher CSF pressure (p < 0.0001) values compared to the sham-operated group. Sixty minutes after SCI or sham operation, CSF pressure was 8.6 ± 0.4 mmHg in the SCI group versus 5.5 ± 0.5 mmHg in the sham-operated group. No dural tears were found after SCI.
CONCLUSION
Our rat model allows SCBF and CSF pressure measurements after induced SCI. After SCI, CSF pressure significantly increases.
摘要
目的
脑脊液（CSF）压力升高可能会使脊髓损伤（SCI）后脊髓缺血恶化。我们建立了一个大鼠模型来研究 CSF 压力与脊髓血流（SCBF）之间的关系。
方法
雄性 Wistar 大鼠在 T10 进行 SCI（n = 7）或假手术（n = 10）。使用激光多普勒测量 SCBF，并通过骶骨导管测量 CSF 压力。通过硬脑膜下亚甲蓝注射（n = 5）和脊髓造影（n = 5）评估硬脑膜完整性。
结果
与假手术组相比，SCI 组的 SCBF 显著降低（p < 0.0001），CSF 压力显著升高（p < 0.0001）。SCI 或假手术后 60 分钟，SCI 组的 CSF 压力为 8.6 ± 0.4mmHg，假手术组为 5.5 ± 0.5mmHg。SCI 后未发现硬脑膜撕裂。
结论
我们的大鼠模型允许在诱导 SCI 后测量 SCBF 和 CSF 压力。SCI 后，CSF 压力显著升高。
相似文献
1
Rat model of spinal cord injury preserving dura mater integrity and allowing measurements of cerebrospinal fluid pressure and spinal cord blood flow.保留硬脑膜完整性并允许测量脑脊液压力和脊髓血流的脊髓损伤大鼠模型。
Eur Spine J. 2013 Aug;22(8):1810-9. doi: 10.1007/s00586-013-2744-2. Epub 2013 Mar 19.
2
Cerebrospinal fluid drainage and induced hypertension improve spinal cord perfusion after acute spinal cord injury in pigs.脑脊液引流和诱导性高血压可改善猪急性脊髓损伤后的脊髓灌注。
Neurosurgery. 2015 Apr;76(4):461-8; discussion 468-9. doi: 10.1227/NEU.0000000000000638.
3
Contrast enhanced ultrasound imaging for assessment of spinal cord blood flow in experimental spinal cord injury.超声造影成像用于评估实验性脊髓损伤中的脊髓血流
J Vis Exp. 2015 May 7(99):e52536. doi: 10.3791/52536.
4
Effect of altering cerebrospinal fluid pressure on spinal cord blood flow.改变脑脊液压力对脊髓血流的影响。
Ann Thorac Surg. 1994 Jul;58(1):112-5. doi: 10.1016/0003-4975(94)91082-0.
5
Secondary spinal cord hypoperfusion of circumscribed areas after injury in rats.大鼠损伤后局限性区域的继发性脊髓低灌注
Neurol Res. 2005 Jun;27(4):403-8. doi: 10.1179/016164105X25180.
6
Effect of norepinephrine on spinal cord blood flow and parenchymal hemorrhage size in acute-phase experimental spinal cord injury.去甲肾上腺素对急性期实验性脊髓损伤中脊髓血流及实质内出血大小的影响。
Eur Spine J. 2014 Mar;23(3):658-65. doi: 10.1007/s00586-013-3086-9. Epub 2013 Nov 14.
7
A Study of Critical Events That Lead to Spinal Cord Injury and the Importance of Rapid Reversal of Surgical Steps in Improving Neurological Outcomes: A Porcine Model.导致脊髓损伤的关键事件研究及快速逆转手术步骤对改善神经预后的重要性：一种猪模型。
Spine (Phila Pa 1976). 2020 Feb 15;45(4):E181-E188. doi: 10.1097/BRS.0000000000003229.
8
Development of a large-animal model to measure dynamic cerebrospinal fluid pressure during spinal cord injury: Laboratory investigation.开发一种大型动物模型来测量脊髓损伤期间的动态脑脊液压力：实验室研究。
J Neurosurg Spine. 2012 Jun;16(6):624-35. doi: 10.3171/2012.3.SPINE11970. Epub 2012 Apr 20.
9
Real-time monitoring of spinal cord blood flow with a novel sensor mounted on a cerebrospinal fluid drainage catheter in an animal model.在动物模型中，使用安装在脑脊液引流导管上的新型传感器对脊髓血流进行实时监测。
J Thorac Cardiovasc Surg. 2014 Oct;148(4):1726-31. doi: 10.1016/j.jtcvs.2014.04.011. Epub 2014 Apr 13.
10
Real-time and spatial quantification using contrast-enhanced ultrasonography of spinal cord perfusion during experimental spinal cord injury.采用对比增强超声成像技术实时和空间定量检测实验性脊髓损伤过程中的脊髓灌注。
Spine (Phila Pa 1976). 2012 Oct 15;37(22):E1376-82. doi: 10.1097/BRS.0b013e318269790f.
引用本文的文献
1
A custom-made weight-drop impactor to produce consistent spinal cord injury outcomes in a rat model.一种定制的重量下降撞击器，用于在大鼠模型中产生一致的脊髓损伤结果。
Transl Neurosci. 2023 May 26;14(1):20220287. doi: 10.1515/tnsci-2022-0287. eCollection 2023 Jan 1.
2
Significance of spinal cord perfusion pressure following spinal cord injury: A systematic scoping review.脊髓损伤后脊髓灌注压的意义：一项系统的范围综述。
J Clin Orthop Trauma. 2022 Sep 11;34:102024. doi: 10.1016/j.jcot.2022.102024. eCollection 2022 Nov.
3
Elevated intraspinal pressure in traumatic spinal cord injury is a promising therapeutic target.创伤性脊髓损伤中升高的脊髓内压力是一个有前景的治疗靶点。
Neural Regen Res. 2022 Aug;17(8):1703-1710. doi: 10.4103/1673-5374.332203.
4
miR-378-3p alleviates contusion spinal cord injury by negatively regulating ATG12.miR-378-3p 通过负向调控 ATG12 缓解挫伤性脊髓损伤。
Int J Exp Pathol. 2021 Aug;102(4-5):200-208. doi: 10.1111/iep.12400. Epub 2021 Oct 28.
5
The spatiotemporal spread of cervical spinal cord contusion injury pathology revealed by 3D in-line phase contrast synchrotron X-ray microtomography.三维共线相位衬度同步辐射 X 射线微断层成像揭示颈脊髓挫伤损伤病理的时空分布。
Exp Neurol. 2021 Feb;336:113529. doi: 10.1016/j.expneurol.2020.113529. Epub 2020 Nov 19.
6
Clinical outcomes of epidural and intradural decompression for treatment of degenerative cervical myelopathy.硬膜外和硬膜内减压治疗退行性颈椎脊髓病的临床结果。
J Int Med Res. 2020 Jun;48(6):300060520929585. doi: 10.1177/0300060520929585.
7
Dynamic changes in intramedullary pressure 72 hours after spinal cord injury.脊髓损伤72小时后髓内压力的动态变化
Neural Regen Res. 2019 May;14(5):886-895. doi: 10.4103/1673-5374.249237.
8
Promotion of neuronal regeneration by using self-polymerized dendritic polypeptide scaffold for spinal cord tissue engineering.利用自聚树枝状多肽支架促进脊髓组织工程中的神经元再生。
J Mater Sci Mater Med. 2017 Dec 14;29(1):6. doi: 10.1007/s10856-017-6010-8.
9
Combinatorial therapy with two pro-coagulants and one osmotic agent reduces the extent of the lesion in the acute phase of spinal cord injury in the rat.两种促凝血剂和一种渗透剂的联合治疗可减轻大鼠脊髓损伤急性期的损伤程度。
Intensive Care Med Exp. 2017 Dec 11;5(1):51. doi: 10.1186/s40635-017-0164-z.
10
In vivo two-photon imaging of axonal dieback, blood flow, and calcium influx with methylprednisolone therapy after spinal cord injury.脊髓损伤后甲基强的松龙治疗对轴突退变、血流和钙内流的体内双光子成像。
Sci Rep. 2015 May 19;5:9691. doi: 10.1038/srep09691.
本文引用的文献
1
The pressure distribution of cerebrospinal fluid responds to residual compression and decompression in an animal model of acute spinal cord injury.脑脊液压力分布对急性脊髓损伤动物模型中的残余压缩和减压作出反应。
Spine (Phila Pa 1976). 2012 Nov 1;37(23):E1422-31. doi: 10.1097/BRS.0b013e31826ba7cd.
2
Real-time and spatial quantification using contrast-enhanced ultrasonography of spinal cord perfusion during experimental spinal cord injury.采用对比增强超声成像技术实时和空间定量检测实验性脊髓损伤过程中的脊髓灌注。
Spine (Phila Pa 1976). 2012 Oct 15;37(22):E1376-82. doi: 10.1097/BRS.0b013e318269790f.
3
Development of a large-animal model to measure dynamic cerebrospinal fluid pressure during spinal cord injury: Laboratory investigation.开发一种大型动物模型来测量脊髓损伤期间的动态脑脊液压力：实验室研究。
J Neurosurg Spine. 2012 Jun;16(6):624-35. doi: 10.3171/2012.3.SPINE11970. Epub 2012 Apr 20.
4
Mechanical indicators of injury severity are decreased with increased thecal sac dimension in a bench-top model of contusion type spinal cord injury.在撞击型脊髓损伤的台面上模型中，随着脊膜囊尺寸的增加，损伤严重程度的机械指标降低。
J Biomech. 2012 Apr 5;45(6):1003-10. doi: 10.1016/j.jbiomech.2012.01.025. Epub 2012 Feb 18.
5
Blood supply and vascular reactivity of the spinal cord under normal and pathological conditions.脊髓在正常和病理条件下的血液供应和血管反应性。
J Neurosurg Spine. 2011 Sep;15(3):238-51. doi: 10.3171/2011.4.SPINE10543. Epub 2011 Jun 10.
6
Spinal cord injury: a systematic review of current treatment options.脊髓损伤：当前治疗选择的系统评价。
Clin Orthop Relat Res. 2011 Mar;469(3):732-41. doi: 10.1007/s11999-010-1674-0.
7
Lumbar cerebrospinal fluid drainage for thoracoabdominal aortic surgery: rationale and practical considerations for management.胸主动脉腹主动脉手术中腰椎脑脊液引流：管理的基本原理和实际考虑。
Anesth Analg. 2010 Jul;111(1):46-58. doi: 10.1213/ANE.0b013e3181ddddd6. Epub 2010 Jun 3.
8
Role of early surgical decompression of the intradural space after cervical spinal cord injury in an animal model.颈椎损伤后硬脊膜内空间早期手术减压的作用：动物模型研究
J Bone Joint Surg Am. 2010 May;92(5):1206-14. doi: 10.2106/JBJS.I.00740.
9
Poisson's ratio and strain rate dependency of the constitutive behavior of spinal dura mater.脊柱硬脑膜本构行为的泊松比和应变率依赖性。
Ann Biomed Eng. 2010 Mar;38(3):975-83. doi: 10.1007/s10439-010-9924-6. Epub 2010 Jan 20.
10
A systematic review of intensive cardiopulmonary management after spinal cord injury.脊髓损伤后强化心肺管理的系统评价。
J Neurotrauma. 2011 Aug;28(8):1479-95. doi: 10.1089/neu.2009.1156. Epub 2010 Apr 8.
Zotero 插件