Department of Biochemistry, Srimad Andavan Arts and Science College, Tiruchirappalli 620005, Tamil Nadu, India.
Singapore Med J. 2009 Mar;50(3):300-2.
This study was designed to scientifically evaluate the effects of an aqueous extract of Muntingia calabura L. (M. calabura), a medicinal herb, on isoproterenol-induced myocardial infarction (MI) in rat models.
Six groups of Wistar albino rats, each comprising six animals, were selected for this study. Group I served as a control, Group II rats were given isoproterenol (20 mg/100g, subcutaneously), and Group III rats were given M. calabura leaf extract (300 mg/kg). Groups IV, V and VI rats were given M. calabura leaf extract (100 mg/kg, 200 mg/kg and 300 mg/kg, respectively) and isoproterenol (20 mg/100g subcutaneously) prior to MI induction. The transaminases (aspartate transaminase and alanine transaminase), lactate dehydrogenase (LDH) and creatine phosphokinase (CK), were estimated in both the serum and heart tissues, and the serum uric acid level was also estimated.
Isoproterenol significantly increased the activities of CK, LDH and the transaminases in serum with a concomitant decrease in these enzymes in tissue. Pretreatment with the aqueous leaf extract of M. calabura at a dose of 300 mg/kg body weight for 30 days had a significant effect on the activities of marker enzymes compared to the other groups. Serum uric acid level, which increased on isoproterenol administration, registered near normal values on treatment with the leaf extract under study.
The study confirms the protective effects of M. calabura leaf extract against isoproterenol-induced biochemical alterations in rats.
本研究旨在科学评估药用植物三叶苦（Muntingia calabura L.）水提物对异丙肾上腺素诱导的大鼠心肌梗死（MI）的影响。
选择 6 组 Wistar 白化大鼠，每组 6 只。第 1 组为对照组，第 2 组大鼠皮下给予异丙肾上腺素（20mg/100g），第 3 组大鼠给予三叶苦叶提取物（300mg/kg）。第 4、5 和 6 组大鼠在诱导 MI 前分别给予三叶苦叶提取物（100mg/kg、200mg/kg 和 300mg/kg）和异丙肾上腺素（20mg/100g 皮下注射）。测定血清和心肌组织中转氨酶（天门冬氨酸转氨酶和丙氨酸转氨酶）、乳酸脱氢酶（LDH）和肌酸磷酸激酶（CK）的活性，以及血清尿酸水平。
异丙肾上腺素显著增加了血清中 CK、LDH 和转氨酶的活性，同时降低了组织中这些酶的活性。用 300mg/kg 体重的三叶苦水提物预处理 30 天，与其他组相比，对标记酶的活性有显著影响。在给予异丙肾上腺素后升高的血清尿酸水平在给予研究用叶提取物后接近正常。
本研究证实了三叶苦叶提取物对异丙肾上腺素诱导的大鼠生化改变的保护作用。
