Hong Jung-Hee, Lee In-Seon
Traditional Microorganism Resources Center, Keimyung University, Dalseo-Gu, Daegu, Republic of Korea.
Biofactors. 2009 Jul-Aug;35(4):380-8. doi: 10.1002/biof.35.
The objectives of this study were to determine the effects of Artemisia capillaris on the viability, antioxidant status, and cytoprotective against oxidative stress induced by hydrogen peroxide in V79 cells. Pretreatment with ethyl acetate fraction increased 363% cell viability compared to the positive control (PC). The ethyl acetate fraction of A. capillaris scavenged intracellular reactive oxygen species (ROS) and increased the activities of cellular antioxidant enzymes like superoxide dismutase (SOD), glutathione peroxidase (GSH-px), and catalase (CAT), and also increased glutathione (GSH) content. It thereby prevented lipid peroxidation which was demonstrated by the inhibition of the formation of thiobarbituric acid reactive substance (TBARS). Furthermore, ethyl acetate fraction reduced the apoptotic cells and cell cycle arrest at G2/M phase induced by H(2)O(2). These findings suggest that A. capillaris ethyl acetate fraction protected V79 cells against H(2)O(2) damage, by enhancing the antioxidative activity.
本研究的目的是确定茵陈蒿对V79细胞活力、抗氧化状态以及对过氧化氢诱导的氧化应激的细胞保护作用。与阳性对照（PC）相比，用乙酸乙酯部位预处理可使细胞活力提高363%。茵陈蒿的乙酸乙酯部位清除细胞内活性氧（ROS），并提高细胞抗氧化酶如超氧化物歧化酶（SOD）、谷胱甘肽过氧化物酶（GSH-px）和过氧化氢酶（CAT）的活性，还增加了谷胱甘肽（GSH）含量。由此防止了脂质过氧化，这通过硫代巴比妥酸反应物质（TBARS）形成的抑制得以证明。此外，乙酸乙酯部位减少了由H₂O₂诱导的凋亡细胞以及G2/M期的细胞周期阻滞。这些发现表明，茵陈蒿乙酸乙酯部位通过增强抗氧化活性保护V79细胞免受H₂O₂损伤。
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