Ma Jiang-Min, Zhou Ting-Jun, Wang Ran, Shan Jing, Wu Yu-Nong, Song Xiao-Ling, Gu Ning, Fan Yuan
Institute of Stomatology, Nanjing Medical University, 136#, Hanzhong Road, 210029, Nanjing, Jiangsu, People's Republic of China.
Eur Arch Otorhinolaryngol. 2014 Dec;271(12):3291-5. doi: 10.1007/s00405-014-2935-4. Epub 2014 Feb 18.
Oral and pharyngeal cancer is the sixth most common cancer worldwide, the 5-year survival rate has not yet increased. A key factor in rates not having improved is the lack of early detection. This study was undertaken to estimate the diagnostic accuracy of brush biopsy with DNA-image cytometry (a noninvasive method) for potentially malignant oral disorders compared with tissue biopsy pathology in China. Exfoliative cells were obtained using a cytobrush cell collector from oral mucosa of 52 subjects, followed by scalpel biopsy from the same region. Nuclear DNA contents (ploidy) were measured after Feulgen restaining, using an automated DNA image cytometer. Exfoliative cytology with DNA-image cytometry and histopathological diagnosis were performed separately at different institutions. Histological investigation was considered the gold standard. We reported that the sensitivity of DNA aneuploidy for the detection of cancer cells in potentially malignant oral disorders was 86.36 %, its specificity was 90.00 %, its positive predictive value was 86.36 %, and its negative predictive value was 90.00 %. Brush biopsy with DNA-image cytometry is a useful method for monitoring potentially malignant oral disorders.
口腔和咽癌是全球第六大常见癌症，其5年生存率尚未提高。生存率未得到改善的一个关键因素是缺乏早期检测。本研究旨在评估在中国，与组织活检病理相比，采用DNA图像细胞术（一种非侵入性方法）的刷检活检对潜在恶性口腔疾病的诊断准确性。使用细胞刷收集器从52名受试者的口腔黏膜获取脱落细胞，随后从同一区域进行手术刀活检。在福尔根复染后，使用自动DNA图像细胞仪测量核DNA含量（倍性）。DNA图像细胞术的脱落细胞学检查和组织病理学诊断在不同机构分别进行。组织学检查被视为金标准。我们报告称，DNA非整倍体对潜在恶性口腔疾病中癌细胞检测的敏感性为86.36%，特异性为90.00%，阳性预测值为86.36%，阴性预测值为90.00%。采用DNA图像细胞术的刷检活检是监测潜在恶性口腔疾病的一种有用方法。
