Cho Won Kyong, Chen Xiong-Yan, Uddin Nazim Mohamad, Rim Yeonggil, Moon Juyeon, Jung Jin-Hee, Shi Chunlin, Chu Hyosub, Kim Suwha, Kim Seon-Won, Park Zee-Yong, Kim Jae-Yean
Division of Applied Life Science (BK21 program), Environmental Biotechnology, National Core Research Center, PMBBRC, Gyeongsang National University, Jinju 660-701, Republic of Korea.
Phytochemistry. 2009 Mar;70(5):570-8. doi: 10.1016/j.phytochem.2009.03.004. Epub 2009 Apr 6.
Commercially, lettuce (Lactuca sativa) is one of the most important leafy vegetables. Lettuce produces a milky latex of variable chemical compositions within its laticifers. As a step toward understanding the main physiological roles of this latex in higher plants, we embarked on its proteomic analysis. We investigated 587 latex proteins that were identified from the lettuce latex using multidimensional protein-identification technology. A bioinformatics analysis showed that the most frequently encountered proteins in the latex were organellar proteins from plastids and mitochondria, followed by nucleic and cytoplasmic proteins. Functional classification of the identified proteins showed that proteins related to metabolism, cell rescue, defense, and virulence were the most abundant in lettuce latex. Furthermore, numerous resistance proteins of lettuce and viral proteins were present in the latex suggesting for the first time a possible function of the lettuce latex in defense or pathogenesis. To the knowledge of the authors, this is the first large-scale proteome analysis of lettuce latex.
在商业上，生菜（莴苣）是最重要的叶菜类蔬菜之一。生菜在其乳汁管中产生化学成分各异的乳状汁液。作为了解这种汁液在高等植物中主要生理作用的第一步，我们着手对其进行蛋白质组分析。我们利用多维蛋白质鉴定技术研究了从生菜汁液中鉴定出的587种乳胶蛋白。生物信息学分析表明，乳胶中最常见的蛋白质是来自质体和线粒体的细胞器蛋白，其次是核酸和细胞质蛋白。对鉴定出的蛋白质进行功能分类表明，与代谢、细胞拯救、防御和毒力相关的蛋白质在生菜汁液中最为丰富。此外，生菜的许多抗性蛋白和病毒蛋白也存在于汁液中，这首次表明生菜汁液在防御或发病机制中可能具有某种功能。据作者所知，这是首次对生菜汁液进行大规模蛋白质组分析。
