Ferrage Fabien, Cowburn David, Ghose Ranajeet
New York Structural Biology Center, New York, New York 10027, USA.
J Am Chem Soc. 2009 May 6;131(17):6048-9. doi: 10.1021/ja809526q.
The steady-state {(1)H}-(15)N NOE experiment is used in most common NMR analyses of backbone dynamics to accurately ascertain the effects of the fast dynamic modes. We demonstrate here that, in its most common implementation, this experiment generates an incorrect steady state in the presence of CSA/dipole cross-correlated relaxation leading to large errors in the characterization of these high-frequency modes. This affects both the quantitative and qualitative interpretation of (15)N backbone relaxation in dynamic terms. We demonstrate further that minor changes in the experimental implementation effectively remove these errors and allow a more accurate interpretation of protein backbone dynamics.
稳态{(1)H}-(15)N NOE实验用于大多数常见的主链动力学NMR分析，以准确确定快速动力学模式的影响。我们在此证明，在其最常见的实施方式中，该实验在存在CSA/偶极子交叉相关弛豫的情况下会产生不正确的稳态，从而导致在表征这些高频模式时出现大的误差。这在动力学方面影响了(15)N主链弛豫的定量和定性解释。我们进一步证明，实验实施中的微小变化有效地消除了这些误差，并允许对蛋白质主链动力学进行更准确的解释。
