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在花椰菜花叶病毒反式激活蛋白存在的情况下多顺反子mRNA的翻译

Translation of a polycistronic mRNA in the presence of the cauliflower mosaic virus transactivator protein.

作者信息

Fütterer J, Hohn T

机构信息

Friedrich Miescher-Institut, Basel, Switzerland.

出版信息

EMBO J. 1991 Dec;10(12):3887-96. doi: 10.1002/j.1460-2075.1991.tb04958.x.

DOI:10.1002/j.1460-2075.1991.tb04958.x
PMID:1935908
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC453126/
Abstract

Polycistronic mRNAs containing an upstream beta-glucuronidase (GUS) and a downstream chloramphenicol acetyltransferase (CAT) reporter open reading frame (ORF) were expressed in transfected plant protoplasts. CAT expression could be strongly induced by coexpression of the cauliflower mosaic virus encoded translation transactivator. Transactivation was abolished when an upstream ORF overlapped the CAT ORF for a long distance. No specific sequence elements were required for transactivation but the presence of a short ORF upstream of the GUS ORF strongly enhanced the process. The inhibitory effect of additional presumed stem structures inserted into various regions of the reporter mRNAs indicates that both ORFs are translated by ribosomes that associate with the RNA at the 5' end and reach the ORFs by a linear migration mechanism.

摘要

含有上游β-葡萄糖醛酸酶（GUS）和下游氯霉素乙酰转移酶（CAT）报告开放阅读框（ORF）的多顺反子mRNA在转染的植物原生质体中表达。花椰菜花叶病毒编码的翻译反式激活因子的共表达可强烈诱导CAT表达。当一个上游ORF与CAT ORF长距离重叠时，反式激活被消除。反式激活不需要特定的序列元件，但GUS ORF上游存在短ORF可强烈增强该过程。插入到报告mRNA不同区域的额外假定茎结构的抑制作用表明，两个ORF都是由在5'端与RNA结合并通过线性迁移机制到达ORF的核糖体翻译的。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6264/453126/2c0b6e82f593/emboj00110-0317-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6264/453126/27771413ccb8/emboj00110-0313-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6264/453126/2aac5f8f288b/emboj00110-0314-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6264/453126/f91b07cb7ffb/emboj00110-0316-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6264/453126/2c0b6e82f593/emboj00110-0317-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6264/453126/27771413ccb8/emboj00110-0313-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6264/453126/2aac5f8f288b/emboj00110-0314-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6264/453126/f91b07cb7ffb/emboj00110-0316-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6264/453126/2c0b6e82f593/emboj00110-0317-a.jpg

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2
In vitro expression of cauliflower mosaic virus genes.在体外用菜花 mosaic 病毒基因表达。
EMBO J. 1988 Feb;7(2):309-17. doi: 10.1002/j.1460-2075.1988.tb02814.x.
3
The sequence of carnation etched ring virus DNA: comparison with cauliflower mosaic virus and retroviruses.康乃馨蚀环病毒 DNA 的序列：与花椰菜花叶病毒和逆转录病毒的比较。
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