Biology Department C016, University of California at San Diego, La Jolla, CA 92093.
Proc Natl Acad Sci U S A. 1988 Feb;85(3):733-7. doi: 10.1073/pnas.85.3.733.
Gene VI of the cauliflower mosaic virus (CaMV) genome encodes a protein (P(66)) in virus-infected plants that accumulates in cytoplasmic inclusion bodies. When a segment of the CaMV genome bearing gene VI is transferred to tobacco plants by the Agrobacterium tumefaciens Ti plasmid, the resulting transgenic plants display viral-like symptoms. Symptoms produced by the DNA from two different viral isolates (CaMV Cabb B-JI and CM1841) were distinct-symptoms from the first were mosaic-like, whereas the other caused uniform bleaching of leaves. That gene VI was responsible for the symptomatic phenotype was demonstrated by showing that symptom production was blocked by deletions and by a frame-shifting linker mutation in gene VI. Furthermore, in primary transformants, there was a strict correlation between the appearance of symptoms and the presence of gene VI product, P(66), detected by immunoblots. Hence, a protein encoded by the CaMV genome produces viral-like symptoms in transgenic tobacco plants.
花椰菜花叶病毒（CaMV）基因组的基因 VI 在感染病毒的植物中编码一种蛋白质（P(66)），该蛋白质在细胞质包涵体中积累。当携带基因 VI 的 CaMV 基因组片段通过根瘤农杆菌 Ti 质粒转移到烟草植物中时，产生的转基因植物表现出类似病毒的症状。来自两种不同病毒分离株（CaMV Cabb B-JI 和 CM1841）的 DNA 产生的症状不同——第一种是镶嵌状的，而另一种则导致叶片均匀白化。基因 VI 负责产生症状表型，这是通过显示基因 VI 中的缺失和移框连接突变阻断了症状的产生而证明的。此外，在初级转化体中，通过免疫印迹检测到基因 VI 产物 P(66)的存在与症状的出现之间存在严格的相关性。因此，CaMV 基因组编码的一种蛋白质在转基因烟草植物中产生类似病毒的症状。
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