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上游开放阅读框在植物细胞多顺反子mRNA翻译中的作用。

Role of an upstream open reading frame in the translation of polycistronic mRNAs in plant cells.

作者信息

Fütterer J, Hohn T

机构信息

Friedrich Miescher-Institute, Basel, Switzerland.

出版信息

Nucleic Acids Res. 1992 Aug 11;20(15):3851-7. doi: 10.1093/nar/20.15.3851.

DOI:10.1093/nar/20.15.3851
PMID:1508670
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC334058/
Abstract

The influence of an upstream small open reading frame (URF) on the translation of two consecutive coding regions on an eukaryotic mRNA was studied. The cis effects of leader length, URF length, the sequences of the URF and neighboring regions, and the trans effects of the Cauliflower mosaic virus transactivator (TAV) were analyzed. Translation efficiency of the immediate downstream open reading frame (ORF) decreased with increasing URF length. Short URFs did not drastically inhibit translation of immediate downstream ORFs but supported far downstream translation in the presence of TAV. In the latter case, the optimal URF length was 30 codons.

摘要

研究了上游小开放阅读框（URF）对真核生物mRNA上两个连续编码区翻译的影响。分析了前导长度、URF长度、URF及其相邻区域序列的顺式效应，以及花椰菜花叶病毒反式激活因子（TAV）的反式效应。紧邻下游开放阅读框（ORF）的翻译效率随URF长度增加而降低。短的URF不会大幅抑制紧邻下游ORF的翻译，但在TAV存在的情况下支持远下游的翻译。在后一种情况下，最佳URF长度为30个密码子。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4af7/334058/9b164abfecda/nar00226-0053-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4af7/334058/9b164abfecda/nar00226-0053-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4af7/334058/9b164abfecda/nar00226-0053-a.jpg

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