Thrombospondin 1 is expressed by mesenchymal cells in mouse post-natal skin and hair follicle development.

Thrombospondin 1 is an extracellular matrix glycoprotein with multiple functions. In the skin, it has been immunolocalized to basement membrane, and its expression increases during embryogenesis and wound healing. Its normal cellular source in the skin is not known, except during wound healing, where macrophages and keratinocytes seem to be the primary source. We have analysed the expression of thrombospondin 1 mRNA in normal mouse skin at different ages by in situ hybridization. It was found that the mRNA is expressed by dermal mesenchymal cells and mature fibroblasts and developmentally regulated during post-natal skin growth and morphogenesis. In adult mouse skin, expression of the thrombospondin is restricted to the mesenchymal cells of hair follicle papilla. These results suggest that the regulation of thrombospondin 1 transcription in mesenchymal cells can play an important role in post-natal skin development. Its mRNA expression is a characteristic of adult dermal papilla cells with a potential role in hair development.