Department of Chemistry MC 111, University of Illinois at Chicago, 845 West Taylor Street, Chicago, IL 60607, USA.
Amino Acids. 2010 Mar;38(3):779-88. doi: 10.1007/s00726-009-0284-1. Epub 2009 Apr 10.
This study investigated the effect of different sampling environments on hemolymph amino acid content of individual Drosophila melanogaster larvae. Hemolymph was collected from individual third instar larvae under cold-anesthetized, awake, and stress conditions. Qualitative and quantitative hemolymph amino acid analyses were performed via capillary electrophoresis with laser-induced fluorescence detection. The hemolymph amino acid concentrations, particularly arginine, glutamate, and taurine, changed significantly depending on the prior-to-sample-collection environments. Hemolymph amino acid analyses of six different Drosophila genotypes including two control genotypes and four mutant alleles were also carried out. Two mutant genotypes with over and under expression of a putative cystine-glutamate exchanger subunit were significantly different from each other with respect to their hemolymph glutamate, glycine, lysine, and taurine levels. Hemolymph amino acid analyses of stressed larvae of two control and two mutant genotypes indicated that behavior-related hemolymph chemical changes are also genotype dependent.
本研究调查了不同采样环境对个体黑腹果蝇幼虫血淋巴氨基酸含量的影响。血淋巴是从处于冷麻醉、清醒和应激状态的个体三龄幼虫中采集的。通过激光诱导荧光检测的毛细管电泳对血淋巴氨基酸进行定性和定量分析。血淋巴氨基酸浓度，特别是精氨酸、谷氨酸和牛磺酸，根据采样前的环境显著变化。还对包括两个对照基因型和四个突变等位基因在内的六个不同黑腹果蝇基因型进行了血淋巴氨基酸分析。两个过表达和低表达假定胱氨酸-谷氨酸交换亚基的突变基因型在血淋巴谷氨酸、甘氨酸、赖氨酸和牛磺酸水平上彼此显著不同。对两个对照和两个突变基因型应激幼虫的血淋巴氨基酸分析表明，与行为相关的血淋巴化学变化也与基因型有关。
