Finke Mark D
Mark D. Finke Inc., Rio Verde, Arizona.
Zoo Biol. 2007 Mar;26(2):105-15. doi: 10.1002/zoo.20123.
Insects contain significant amounts of fiber as measured by crude fiber, acid detergent fiber (ADF) or neutral detergent fiber (NDF). It has always been assumed that the fiber in insects represents chitin based on the structural similarity between cellulose and chitin and the fact that the ADF fraction from insects contains nitrogen. In this study, a number of insect species that are raised commercially as food for insectivores were analyzed for moisture, crude protein (nitrogen x 6.25), fat, ash, NDF, ADF, and amino acids. Additionally, the ADF fraction was analyzed for nitrogen and amino acids to determine if proteins might be present in the ADF fraction. The ADF fraction contained a significant amount of amino acids accounting for 9.3-32.7% of the ADF (by weight). The presence of amino acids in the ADF fraction means that using ADF to estimate insect chitin results in an overestimation of insect chitin content. Using ADF adjusted for its amino acid content, the estimated chitin content of these insect species ranged from 2.7-49.8 mg/kg (as is) and 11.6-137.2 mg/kg (dry matter basis). Additionally, these data suggest that for the species measured here the amount of chitin nitrogen is quite small (as a % of total nitrogen) and that crude protein (nitrogen x 6.25) provides a reasonable estimate of the true protein for most species of insects. Zoo Biol 0:1-11, 2007. (c) 2007 Wiley-Liss, Inc.
通过粗纤维、酸性洗涤纤维（ADF）或中性洗涤纤维（NDF）测定，昆虫含有大量纤维。基于纤维素和几丁质之间的结构相似性以及昆虫的ADF部分含有氮这一事实，一直以来人们都认为昆虫中的纤维代表几丁质。在本研究中，对一些作为食虫动物食物进行商业养殖的昆虫种类进行了水分、粗蛋白（氮×6.25）、脂肪、灰分、NDF、ADF和氨基酸分析。此外，对ADF部分进行了氮和氨基酸分析，以确定ADF部分是否可能存在蛋白质。ADF部分含有大量氨基酸，占ADF的9.3 - 32.7%（按重量计）。ADF部分中氨基酸的存在意味着使用ADF来估计昆虫几丁质会导致对昆虫几丁质含量的高估。使用根据其氨基酸含量调整后的ADF，这些昆虫种类的估计几丁质含量范围为2.7 - 49.8毫克/千克（原样）和11.6 - 137.2毫克/千克（干物质基础）。此外，这些数据表明，对于此处测量的物种，几丁质氮的量相当小（占总氮的百分比），并且粗蛋白（氮×6.25）为大多数昆虫物种的真实蛋白质提供了合理估计。《动物学杂志》0:1 - 11，2007年。（c）2007威利 - 利斯公司。
